Col2a1^tm2.1Mcali
Targeted Allele Detail

Summary

• Symbol: Col2a1^tm2.1Mcali
• Name: collagen, type II, alpha 1; targeted mutation 2.1, Audrey McAlinden
• MGI ID: MGI:5638780
• Synonyms: Col2a1-mIIC
• Gene: Col2a1 Location: Chr15:97873483-97902525 bp, - strand Genetic Position: Chr15, 53.97 cM
• Alliance: Col2a1^tm2.1Mcali page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:215404
• Parent Cell Line: EDJ22 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Modified isoform(s))
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 2 was replaced with one in which mutations (AGGTAT to CACTAC) altered the 5' splice site abolishing production of the type 2c isoform. Cre-mediated recombination removed the floxed neomycin resistance cassette inserted downstream of the modified exon 2. (J:215404)

Expression

In Mice Carrying this Mutation:

98 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col2a1 Mutation: 70 strains or lines available

References

• Original: J:215404 Hering TM, et al., Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2. Matrix Biol. 2014 Jun;36:51-63
• All: 1 reference(s)