Antibody:	Anti-Aire
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Present	Regionally restricted	2C Merge right , 2C Aire	Aire localized in mature medullary thymic epithelial cells. Expression was observed in a vesicle- like localization in the nucleus that colocalized with the Tbata protein.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Present	Regionally restricted	3A Merge top , 3A Aire	Aire staining in mature medullary thymic epithelial cells co-localizes with Krt5, a medullary thymic epithelial cell marker, in those cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Present	Regionally restricted	3B Aire left , 3B Merge left	Aire staining in mature medullary thymic epithelial cells co-localizes with Krt5, a medullary thymic epithelial cell marker, in those cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Absent		3B Merge center , 3B Aire center

Structure	Cell Type	Level	Pattern	Image	Note
TS28: thymus medulla epithelium	medullary thymic epithelial cell	Weak	Regionally restricted	3B Aire right , 3B Merge right	Aire staining in mature medullary thymic epithelial cells was significantly decreased in the mutant compared with wild type.