Gene Expression Data
Assay Details
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Assay
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Reference:
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J:157679
Saade M, et al., Spatial (Tbata) expression in mature medullary thymic epithelial cells. Eur J Immunol. 2010 Feb;40(2):530-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7714944
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Gene symbol:
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Tbata
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Gene name:
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thymus, brain and testes associated
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Results
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Specimen
1B E12.5:
embryonic day 12.5
(more  )
Note: Triple labeled: red - Tbata; blue - Krt5 (assay MGI:7715128); green - Krt8 (assay MGI:7715127).
Specimen
1B E12.5:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: red - Tbata; blue - Krt5 (assay MGI:7715128); green - Krt8 (assay MGI:7715127).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thymus primordium |
Present |
Regionally restricted |
1B Merge E12.5
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1B Spatial E12.5
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Expression was detected in the whole epithelium where it overlapped with Krt5 and Krt8.
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Specimen
1B E15.5:
embryonic day 15.5
(more )
Note: Triple labeled: red - Tbata; blue - Krt5 (assay MGI:7715128); green - Krt8 (assay MGI:7715127).
Specimen
1B E15.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Triple labeled: red - Tbata; blue - Krt5 (assay MGI:7715128); green - Krt8 (assay MGI:7715127).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: thymus primordium |
epithelial cell of thymus |
Present |
Regionally restricted |
1B Merge E15.5
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1B Spatial E15.5
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Smaller clusters of thymic epithelial cells were identified as Krt8+Krt5-, as well as Krt8-Krt5+. Expression of Tbata was detected in both sets of clusters. Additionally, some Tbata+ cells were Krt8+Krt5+.
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Specimen
1C E12.5:
embryonic day 12.5
(more )
Note: Double labeled: red - Tbata; green - MTS24.
Specimen
1C E12.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - MTS24.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: thymus primordium |
Present |
Regionally restricted |
1C Spatial E12.5
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1C Merge E12.5
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Expression colocalized with the epithelial progenitor cell marker MTS24 and was found in some MTS24-negative population.
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Specimen
1C E15.5:
embryonic day 15.5
(more )
Note: Double labeled: red - Tbata; green - MTS24.
Specimen
1C E15.5:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - MTS24.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: thymus primordium |
epithelial cell of thymus |
Present |
Regionally restricted |
1C Merge E15.5
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1C Spatial E15.5
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Expression colocalized with the epithelial progenitor cell marker MTS24.
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| TS23: thymus primordium |
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Present |
Regionally restricted |
1C Spatial E15.5
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1C Merge E15.5
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Expression was found in some MTS24-negative population.
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Specimen
1C Adult:
postnatal adult
(more )
Note: Double labeled: red - Tbata; green - MTS24.
Specimen
1C Adult:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - MTS24.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
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1C Merge Adult
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1C Spatial Adult
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Expression colocalized with MTS24+ population of medullary thymic epithelial cells.
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Specimen
1D C57BL/6J:
postnatal adult
(more )
Specimen
1D C57BL/6J:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus subcapsular epithelium |
epithelial cell of thymus |
Present |
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1D C57BL/6J bottom
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Expressing thymic epithelial cells are mainly concentrated in the subcapsular region of the adult thymus.
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| TS28: thymus cortex epithelium |
cortical thymic epithelial cell |
Present |
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1D C57BL/6J bottom
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Expression in the cortex is diluted due to the presence of thymocytes.
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Specimen
1D CD3epsilon-/-:
(close )
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Genetic Background:
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C57BL/6-Cd3etm1Mal
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Mutant Allele(s):
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Cd3etm1Mal/Cd3etm1Mal
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus epithelium |
epithelial cell of thymus |
Present |
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1D CD3e-/- bottom
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Expression showed a differential distribution in the mutant.
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Specimen
2A upper:
postnatal adult
(more )
Note: Double labeled: red - Tbata; green - Krt8 (assay MGI:7715127).
Specimen
2A upper:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - Krt8 (assay MGI:7715127).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus cortex epithelium |
cortical thymic epithelial cell |
Present |
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2A Merge top
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2A Spatial top
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2A inset
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Expression colocalized with Krt8, a cortical thymic epithelial cell marker. It was seen in both nuclear and cytosolic compartments in the cortex.
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Specimen
2A lower:
postnatal adult
(more )
Note: Double labeled: red - Tbata; green - MTS10.
Specimen
2A lower:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - MTS10.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
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2A Spatial bottom
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2A Merge middle
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2A Spatial middle
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2A Merge bottom
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Expression colocalized with MTS10, a medullary thymic epithelial cell marker. It was localized in the nucleus in the medulla.
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Specimen
2C left:
postnatal adult
(more )
Note: Double labeled: red - Tbata; green - UEA1.
Specimen
2C left:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - UEA1.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
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2C Spatial left
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2C Merge left
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Expression colocalized with UEA1, a medullary thymic epithelial cell marker.
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Specimen
2C right:
postnatal adult
(more )
Note: Double labeled: red - Tbata; green - Aire (assay MGI:7715225).
Specimen
2C right:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Tbata; green - Aire (assay MGI:7715225).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
Regionally restricted |
2C Merge right
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2C Spatial right
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Expression was observed in a vesicle- like localization in the nucleus that colocalized with the Aire protein, which localizes in mature medullary thymic epithelial cells.
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Specimen
3A:
postnatal adult
(more )
Note: Double labeled: green - Tbata; red - MTS10.
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Tbata; red - MTS10.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
Regionally restricted |
3A Merge bottom
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3A Spatial
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Expression overlapped with MTS10, a medullary thymic epithelial cell marker. Expression was found in ~16% of mature medullary thymic epithelial cells that were also Aire+.
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Specimen
3C WT:
postnatal adult
(more )
Note: Double labeled: green - Tbata; red - MTS10.
Specimen
3C WT:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Tbata; red - MTS10.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Present |
Regionally restricted |
3C Spatial left
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3C Merge left
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Expression overlapped with MTS10, a medullary thymic epithelial cell marker.
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Specimen
3C Aire-/-:
postnatal adult; Airetm2Ghr/ Airetm2Ghr
(more )
Note: Double labeled: green - Tbata; red - MTS10.
Specimen
3C Aire-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Airetm2Ghr/Airetm2Ghr
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Tbata; red - MTS10.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Weak |
Regionally restricted |
3C Merge center
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3C Spatial center
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A 28-fold reduction of staining was observed in the mutant.
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Specimen
3C RANKL-/-:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Tnfsf11tm1Pngr/Tnfsf11tm1Pngr
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: green - Tbata; red - MTS10.
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: thymus medulla epithelium |
medullary thymic epithelial cell |
Weak |
Regionally restricted |
3C Merge right
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3C Spatial right
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A 15-fold reduction of staining was observed in the mutant.
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