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Gene Expression Data
Assay Details
Assay
Reference: J:157679 Saade M, et al., Spatial (Tbata) expression in mature medullary thymic epithelial cells. Eur J Immunol. 2010 Feb;40(2):530-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7715128
Gene symbol: Krt5
Gene name: keratin 5
Antibody: Anti-K5
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1B E12.5: embryonic day 12.5 (more )
Note: Triple labeled: blue - Krt5; green - Krt8 (assay MGI:7715127); red - Tbata (assay MGI:7714944).
Structure Level Pattern Image Note
TS20: thymus primordium Present 1B K5 E12.5 , 1B Merge E12.5 Expression was detected in the whole epithelium where it overlapped with Krt8 and Tbata.
Specimen 1B E15.5: embryonic day 15.5 (more )
Note: Triple labeled: blue - Krt5; green - Krt8 (assay MGI:7715127); red - Tbata (assay MGI:7714944).
Structure Cell Type Level Pattern Image Note
TS23: thymus primordium epithelial cell of thymus Present 1B Merge E15.5 , 1B K5 E15.5 Smaller clusters of Krt8-Krt5+ cells were interspersed among Krt8+Krt5- subsets. Some Krt8+Krt5+ cells were maintained. Staining overlapped with Tbata.
Specimen 3A: postnatal adult (more )
Note: Double labeled: red - Krt5; green - Aire (assay MGI:7715225).
Structure Cell Type Level Pattern Image Note
TS28: thymus medulla epithelium medullary thymic epithelial cell Present 3A K5 , 3A Merge top Krt5 is a marker for medullary thymic epithelial cells. Aire staining in mature medullary thymic epithelial cells co-localizes with Krt5 in those cells.
Specimen 3B WT: postnatal adult (more )
Note: Double labeled: red - Krt5; green - Aire (assay MGI:7715225).
Structure Cell Type Level Pattern Image Note
TS28: thymus medulla epithelium medullary thymic epithelial cell Present 3B Merge left , 3B K5 left Krt5 is a marker for medullary thymic epithelial cells. Aire staining in mature medullary thymic epithelial cells co-localizes with Krt5 in those cells.
Specimen 3B Aire-/-: postnatal adult; Airetm2Ghr/Airetm2Ghr (more )
Note: Double labeled: red - Krt5; green - Aire (assay MGI:7715225).
Structure Cell Type Level Pattern Image Note
TS28: thymus medulla epithelium medullary thymic epithelial cell Present 3B K5 center , 3B Merge center Krt5 is a marker for medullary thymic epithelial cells.
Specimen 3B RANKL-/-: postnatal adult; Tnfsf11tm1Pngr/Tnfsf11tm1Pngr (more )
Note: Double labeled: red - Krt5; green - Aire (assay MGI:7715225).
Structure Cell Type Level Pattern Image Note
TS28: thymus medulla epithelium medullary thymic epithelial cell Present 3B K5 right , 3B Merge right Krt5 is a marker for medullary thymic epithelial cells.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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