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Gene Expression Data
Assay Details
Assay
Reference: J:195264 Vincentz JW, et al., Twist1 Controls a Cell-Specification Switch Governing Cell Fate Decisions within the Cardiac Neural Crest. PLoS Genet. 2013 Mar;9(3):e1003405
Assay type: RNA in situ
MGI Accession ID: MGI:7643419
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Probe: Ascl1 probe
Probe preparation: Antisense labelled with digoxigenin RNA
Results
Specimen 2G: embryonic day 11.5 (more )
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Absent 2G
Specimen 2H: embryonic day 11.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS19: outflow tract endocardial cushion neural crest cell Present Regionally restricted 2H Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Ascl1 was detected in the aggregates.
Specimen 3C: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Absent 3C
Specimen 3D: embryonic day 11.5; Twist1tm1Bhr/Twist1tm2Bhr, Hand1tm1.1(EGFP/cre)Abfi/Hand1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Regionally restricted 3D Conditional ablation of Twist1 produces abnormal cellular aggregates. Expression of Ascl1 was detected in the aggregates.
Specimen 7A: embryonic day 11.5; Twist1Ska10/Twist1Ska10 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Regionally restricted 7A Robust aggregates of Ascl1-positive cells were observed.
Specimen 7B: embryonic day 11.5; Phox2btm1Jbr/Phox2b+, Twist1Ska10/Twist1Ska10 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Present Regionally restricted 7B Phox2b<lacz/+> haploinsufficiency tended to reduce the size of aggregates of Ascl1-positive cells.
Specimen 7C: embryonic day 11.5; Phox2btm1Jbr/Phox2btm1Jbr, Twist1Ska10/Twist1Ska10 (more )
Structure Level Pattern Image Note
TS19: outflow tract endocardial cushion Absent 7C
Specimen S3C: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: aortico-pulmonary spiral septum Absent S3C
TS17: outflow tract endocardial cushion Absent S3C
Specimen S3D: embryonic day 10.5; H2az2Tg(Wnt1-cre)11Rth/0, Twist1tm1Bhr/Twist1tm2Bhr (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: aortico-pulmonary spiral septum Present Regionally restricted S3D Ectopic expression was observed in the conditional mutant at E10.5.
TS17: outflow tract endocardial cushion Present Regionally restricted S3D Ectopic expression was observed in the conditional mutant at E10.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory