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Gene Expression Data
Assay Details
Assay
Reference: J:347585 Leon NY, et al., Y chromosome damage underlies testicular abnormalities in ATR-X syndrome. iScience. 2024 May 17;27(5):109629
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7628670
Gene symbol: Gata4
Gene name: GATA binding protein 4
Antibody: anti-GATA-4 (sc-25310)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Assay notes: Two Gata4 antibodies were described, but the one used in this assay was not detailed. Anti-GATA4 name MGI:MGI:5431441 may have been used in this assay.
Results
Specimen 1A Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Atrx (assay MGI:7628671).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1A Control (merged) , 1A Control GATA4 Diffuse expression throughout nucleus. 10% of nuclei contain a single nuclear speckle (Gata4 foci) frequently located near periphery of nucleus.
Specimen 1A ScAttrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Atrx (assay MGI:7628671).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1A ScAtrxKO GATA4 , 1A ScAtrxKO (merged) Diffuse expression throughout nucleus. 26% of nuclei contain a single nuclear speckle (Gata4 foci) frequently located near periphery of nucleus. Some foci significantly larger compared to control.
Specimen S1 Control: embryonic day 17.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Atrx (assay MGI:7628671).
Structure Cell Type Level Pattern Image Note
TS25: Sertoli cell Sertoli cell Present Regionally restricted S1_GATA4 , S1_GATA/ATRX , S1_DAPI/GATA4/ATRX Gata4 foci co-localized in nuclear speckle with strong Atrx expression.
Specimen 1B Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Zfpm2 (assay MGI:7628672).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1B Control GATA4 , 1B Control (merged) Expression co-localized with FOG2 (Zfpm2B) expression in both control and mutant Sertoli cells.
Specimen 1B ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Zfpm2 (assay MGI:7628672).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1B ScAtrxKO (merged) , 1B ScAtrxKO GATA4 Expression co-localized with FOG2 (Zfpm2B) expression in both control and mutant Sertoli cells.
Specimen 1C Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Daxx (assay MGI:7628673).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1C Control GATA4 , 1C Control (merged) Expression co-localized with Daxx expression in control Sertoli cells.
Specimen 1C ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Daxx (assay MGI:7628673).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 1C ScAtrxKO GATA4 , 1C ScAtrxKO (merged)
Specimen 1D Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Pml (assay MGI:7628674).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1D Control (merged) , 1D Control GATA4 17% of Gata4 foci co-localized with one specific promyelocytic leukemia nuclear body (PML NB); rest were PML-negative.
Specimen 1D ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Pml (assay MGI:7628674).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 1D ScAtrxKO GATA4 , 1D ScAtrxKO (merged) 77% of regular sized and 94% of giant Gata4 foci co-localized with PML NBs.
Specimen 2A Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - PH2.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 2A Control More Gata4 foci-positive Sertoli cells at G2/M (44.6%) compared to entire Sertoli cell population.
Specimen 2A ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - PH2.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present Regionally restricted 2A ScatrxKO Sertoli cells with giant Gata4 foci were all at G2/M.
Specimen 3A Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - H2ax (assay MGI:7628675).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 3A Control (merged) , 3A Control GATA4
Specimen 3A ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - H2ax (assay MGI:7628675).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 3A ScAtrxKO (merged) , 3A ScAtrxKO GATA4
Specimen S2 Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Trp53bp1 (assay MGI:7628676).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present S2_Control GATA4 , S2_ Control (merged)
Specimen S2 ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Trp53bp1 (assay MGI:7628676).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present S2_ScAtrxKO GATA4 , S2_ScAtrxKO (merged)
Specimen 4A Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - PH2.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 4A Control GATA4 , 4A Control (merged) 86% of Gata4 foci were PH3-positive.
Specimen 4A ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - PH2.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 4A ScAtrxKO (merged) , 4A ScAtrxKO GATA4
Specimen 5 Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Cbx5 (assay MGI:7628677).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 5_Control GATA4 , 5_Control (merged)
Specimen 5 ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Cbx5 (assay MGI:7628677).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 5_ScAtrxKO GATA4 , 5_ScAtrxKO (merged)
Specimen 6A Control (left): embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Cenpa (assay MGI:7628678).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6A Control GATA4/CENPA
Specimen 6A ScAtrxKO (left): embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Cenpa (assay MGI:7628678).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6A ScAtrxKO GATA4/CENPA
Specimen 6A Control (right): embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4; green - Terf1 (assay MGI:7628679).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6A Control GATA4/TERF1 , 6A Control (inset)
Specimen 6A ScAtrxKO (right): embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4; green - Terf1 (assay MGI:7628679).
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6A ScAtrxKO GATA4/TERF1 , 6A ScAtrxKO (inset)
Specimen 6B I. Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6B I. Control (merged) , 6B I. Control GATA4
Specimen 6B I. ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6B I. ScAtrxKO (merged) , 6B I. ScAtrxKO GATA4
Specimen 6B II. Control: embryonic day 16.5; Atrxtm1Rjg/Y (more )
Note: Double labeled: red - Gata4.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6B II. Control
Specimen 6B II. ScAtrxKO: embryonic day 16.5; Atrxtm1Rjg/Y, Plekha5Tg(AMH-cre)1Flor/0 (more )
Note: Conditional mutant. Double labeled: red - Gata4.
Structure Cell Type Level Pattern Image Note
TS24: Sertoli cell Sertoli cell Present 6B II. ScAtrxKO
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03/18/2025
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