Gene Expression Data
Assay Details
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Assay
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Reference:
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J:291486
Daniel E, et al., Cyp26b1 is an essential regulator of distal airway epithelial differentiation during lung development. Development. 2020 Feb 20;147(4):dev181560
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7623787
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Gene symbol:
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Lamp3
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Gene name:
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lysosomal-associated membrane protein 3
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Results
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Specimen
S3E:
embryonic day 18.5
(more  )
Note: Triple labeled: green - Lamp3; white - Cdh1 (assay MGI:7623789); red - Sftpc (assay MGI:7623780).
Specimen
S3E:
(close  )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Lamp3; white - Cdh1 (assay MGI:7623789); red - Sftpc (assay MGI:7623780).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
type II pneumocyte |
Present |
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S3E'
,
S3E''
,
S3E
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The vast majority of AT2 cells express both Lamp3 and Sftpc.
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Specimen
S3F:
(close )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Mutant Allele(s):
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Cyp26b1em1Oncl/Cyp26b1em1Oncl
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Lamp3; white - Cdh1 (assay MGI:7623789); red - Sftpc (assay MGI:7623780).
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
type II pneumocyte |
Present |
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S3F'
,
S3F
,
S3F''
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Cyp26b1-/- lungs had an increase in AT2 cells compared with control littermates. Co-staining of AT2 cell markers Sftpc and Lamp3 confirmed the result.
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Specimen
not shown +/+:
embryonic day 18.5
(more )
Specimen
not shown +/+:
(close )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
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Expression was detected in distal lung epithelium. Lamp3+ cells could be Sox9-immunopositive or Sox9-negative.
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Specimen
not shown -/-:
(close )
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Genetic Background:
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involves: C57BL/6J * CD-1
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Mutant Allele(s):
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Cyp26b1em1Oncl/Cyp26b1em1Oncl
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
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There was an increase in Sox9+/Lamp3+ distal epithelium cells in the Cyp26b1-/- lungs. The number of Sox9-/Lamp3+ cells was not significantly changed.
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Analysis Tools