Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:291486
Daniel E, et al., Cyp26b1 is an essential regulator of distal airway epithelial differentiation during lung development. Development. 2020 Feb 20;147(4):dev181560
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:7623780
|
Gene symbol:
|
Sftpc
|
Gene name:
|
surfactant associated protein C
|
|
Results
|
Specimen
3D:
embryonic day 18.5
(more )
Specimen
3D:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
3E:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Mutant Allele(s):
|
Cyp26b1em1Oncl/Cyp26b1em1Oncl
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
type II pneumocyte |
Present |
|
3E'
,
3E
|
Cyp26b1-/- lungs had an increase in AT2 cells compared with control littermates.
|
Specimen
4K:
embryonic day 16.5
(more )
Specimen
4K:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung epithelium |
Present |
Regionally restricted |
4K
|
Expression was detected in AT2 progenitors.
|
Specimen
4L:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Mutant Allele(s):
|
Cyp26b1em1Oncl/Cyp26b1em1Oncl
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung epithelium |
Present |
Regionally restricted |
4L
|
Immunostaining revealed no overt qualitative difference in AT2 progenitor patterning.
|
Specimen
S3E:
embryonic day 18.5
(more )
Note: Triple labeled: red - Sftpc; white - Cdh1 (assay MGI:7623789); green - Lamp3 (assay MGI:7623787).
Specimen
S3E:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Triple labeled: red - Sftpc; white - Cdh1 (assay MGI:7623789); green - Lamp3 (assay MGI:7623787).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
type II pneumocyte |
Present |
|
S3E
,
S3E''
|
The vast majority of AT2 cells express both Lamp3 and Sftpc.
|
Specimen
S3F:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Mutant Allele(s):
|
Cyp26b1em1Oncl/Cyp26b1em1Oncl
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Triple labeled: red - Sftpc; white - Cdh1 (assay MGI:7623789); green - Lamp3 (assay MGI:7623787).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
type II pneumocyte |
Present |
|
S3F
,
S3F''
|
Cyp26b1-/- lungs contain an increased in AT2 cells compared with control littermates. Co-staining with Lamp3, an additional marker of AT2 cells confirmed the result.
|
Specimen
S4H:
embryonic day 18.5
(more )
Specimen
S4H:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
S4I:
(close )
Genetic Background:
|
involves: C57BL/6J * CD-1
|
Mutant Allele(s):
|
Cyp26b1em1Oncl/Cyp26b1em2Oncl
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: lung epithelium |
type II pneumocyte |
Present |
|
S4I
,
S4I'
|
There was an increase in Sftpc+ AT2 cells in the Cyp26b1-/delta10 lungs.
|
|