Antibody:	anti-Pax6
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Two antibodies were listed: MGI:7621464 and MGI:7621467; the antibody used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	1H boxed bottom right , 1H left , 1H boxed bottom left	Expression was detected in radial glial progenitor cells (Pax6+) and cells in the transition stage between radial glial progenitor cells/intermediate progenitor cells (Eomes+/Pax6+). There was co-localization with Esco2 in both Pax6+ and Eomes+/Pax6+ cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6A Control	Expression was detected in radial glial progenitor cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6A ESCO2cKO	Expression was detected in radial glial progenitor cells. There was some loss of cells in the dorsal and lateral areas of the conditional mutant cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		6C Control high mag middle left , 6C Control high mag top left	Expression was detected in radial glial progenitor cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		6C ESCO2cKO high mag top right , 6C ESCO2cKO high mag middle right	In the conditional mutant, expression was lower in the lateral area of the cortex. Co-expression with Casp3 and Eomes indicated there was increased apoptosis in cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+) and in radial glial progenitor cells (Pax6+).