Antibody:	anti-Casp3
Detection system:	Secondary antibody coupled to Alexa Fluor

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Ambiguous		6C Control low mag , 6C Control high mag lower left , 6C Control high mag top left , 6C Control high mag middle left

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6C ESCO2cKO low mag , 6C ESCO2cKO high mag middle right , 6C ESCO2cKO high mag top right , 6C ESCO2cKO high mag lower right	The ESCO2cKO mutant cortex demonstrated increased apoptosis. It appeared milder in the lateral area of the cortex than in the medial or dorsal areas. The mutant lateral cortex apoptotic cells were mostly found in the basal side. Co-immunostaining indicated that increased apoptosis was mainly in intermediate progenitor cells (Eomes+) and and cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+). A lesser extent of apoptosis was seen in radial glia progenitor cells (Pax6+).

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Single cells	S2A high mag left , S2A high mag right , S2A low mag , S2A high mag middle	A few Casp3+ apoptotic cells were noted. Closed arrows indicate cells that were Eomes+ intermediate progenitor cells. Open arrow indicates an Eomes-negative apoptotic cell.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex	cerebral cortex neuron	Ambiguous		S6C Control high mag

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cerebral cortex	cerebral cortex neuron	Present	Regionally restricted	S6C ESCO2cKO high mag	Apoptosis was seen in postmitotic neurons of the ESCO2cKO mutant cortex.