Antibody:	anti-Tbr2
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Two antibodies were listed: MGI:7621461 and MGI:7621468; the antibody used in this assay was not specified.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	1A	Expression was detected in intermediate progenitor cells.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Regionally restricted	1H left , 1H boxed top right , 1H boxed bottom right	Expression was detected in intermediate progenitor cells (Eomes+) and cells in the transition stage between radial glial cells/intermediate progenitor cells (Eomes+/Pax6+). There was co-localization with Esco2 in both Eomes+ and Eomes+/Pax6+ cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6A Control	Expression was detected in intermediate progenitor cells.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6A ESCO2cKO	Expression was detected in intermediate progenitor cells. In the conditional mutant, expression was lost in the medial and dorsal areas of the cortex and decreased in the lateral area of the cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6C Control high mag lower left , 6C Control high mag top left	Expression was detected in intermediate progenitor cells in the lateral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	6C ESCO2cKO high mag lower right , 6C ESCO2cKO high mag top right	In the conditional mutant, expression was lower in the lateral area of the cortex. Co-expression with Casp3 and Pax6 indicated that increased apoptosis was mainly in intermediate progenitor cells (Eomes+) and and cells in transition stage between radial glia progenitor cells/intermediate progenitor cells (Eomes+/Pax6+).

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex	Present	Regionally restricted	S2A high mag middle , S2A high mag left , S2A low mag , S2A high mag right	Expression was detected in intermediate progenitor cells. A few Casp3+ apoptotic cells were noted.