Probe:	srGAP3 cDNA
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present		1C
TS19: telencephalon ventricular layer	Present		1C
TS19: trigeminal ganglion	Present		1C
TS19: telencephalon	Present	Regionally restricted	1C	Expression is in the preplate.
TS19: eye	Present	Regionally restricted	1C	Expression is in the optic pit.
TS19: midbrain roof plate	Present	Regionally restricted	1C	Expression is in the superior colliculus differentiating zone.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present		1F
TS21: cerebral cortex intermediate zone	Weak		1F
TS21: cortical subplate	Weak		1F

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Strong		1I
TS24: cerebral cortex intermediate zone	Present		1I
TS24: cerebral cortex ventricular layer	Present		1I

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		1L
TS28: cortical layer III	Present		1L
TS28: cortical layer IV	Present		1L
TS28: cortical layer V	Present		1L
TS28: cortical layer VI	Present		1L
TS28: cortical layer I	Absent		1L

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Strong	Regionally restricted	2C	Expression is in the hippocampal neuroepithelium.
TS21: thalamus	Present		2C	Expression is in the dorsal and ventral thalamus.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Strong		2F
TS27: hippocampus CA3	Strong		2F
TS27: dentate gyrus	Present		2F
TS27: hippocampus	Present		2F
TS27: thalamus	Present		2F	Expression was in the ventral thalamus.
TS27: dorsal thalamus	Weak		2F

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present		2I
TS28: dorsal lateral geniculate nucleus	Present		2I
TS28: hippocampus	Present		2I
TS28: hippocampus CA1	Present		2I
TS28: hippocampus CA3	Present		2I
TS28: ventral lateral geniculate nucleus	Present		2I

Structure	Level	Pattern	Image	Note
TS21: midbrain roof plate	Present	Non-Uniform	3C	Expression is stronger in the superior colliculus differentiating zone and throughout the inferior colliculus.
TS21: cerebellum	Present	Regionally restricted	3C	Expression is in the cerebellar plate and in the cerebellar nuclei differentiating zone.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present	Regionally restricted	3C'	Expression is in the granule cells.

Structure	Level	Pattern	Image	Note
TS24: superior colliculus	Present		3F
TS24: inferior colliculus	Present		3F
TS24: tectum	Present	Regionally restricted	3F	Expression is in the inferior colliculus differentiating zone.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Present		3I
TS27: superior colliculus	Present		3I
TS27: inferior colliculus	Present	Regionally restricted	3I	Expression is in a region of the inferior colliculus.
TS27: cerebellum external granule cell layer	Weak		3I
TS27: cerebellum granule cell layer	Weak		3I

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		3L
TS28: cerebellum external granule cell layer	Present		3L
TS28: cerebellum granule cell layer	Present		3L
TS28: deep cerebellar nucleus	Present	Regionally restricted	3L
TS28: cerebellum molecular layer	Absent		3L

Structure	Cell Type	Level	Pattern	Image	Note
TS19: neural tube floor plate		Present		4C
TS19: neural tube roof plate		Present		4C
TS19: neural tube	commissural neuron	Present	Regionally restricted	4C	Expression is in a stream of of commissural neurons positioned medially, in a dorsal region of the cord around the roof plate, and in the ventral horns. Weak expression in the ventral motor columns.
TS19: dorsal root ganglion		Weak		4C

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Strong		4F	Expression was particularly strong in the ependymal layer.
TS20: dorsal root ganglion	Strong		4F

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Present		4I	Expression was particularly strong in the ependymal layer.
TS21: dorsal root ganglion	Present		4I

Structure	Cell Type	Level	Pattern	Image	Note
TS22: main olfactory bulb mitral cell layer		Strong		5C
TS22: vomeronasal organ		Present		5C
TS22: main olfactory bulb layer	neuron	Present	Scattered	5C	Expression is in some scattered neurons of the ganglion layer.
TS22: olfactory epithelium		Absent		5C

Structure	Level	Pattern	Image	Note
TS27: main olfactory bulb mitral cell layer	Present		5F
TS27: main olfactory bulb granule cell layer	Weak		5F
TS27: olfactory epithelium	Absent		5F

Structure	Level	Pattern	Image	Note
TS20: neural retina	Present	Regionally restricted	6C	Expression is in the retinal ganglion cell layer and the neuroblastic layer

Structure	Level	Pattern	Image	Note
TS24: retina ganglion cell layer	Present		6F
TS24: retina nuclear layer	Present		6F

Structure	Level	Pattern	Image	Note
TS19: dorsal root ganglion	Present
TS19: nasal pit	Present
TS19: neural retina	Present	Regionally restricted		Expression is in the retinal ganglion layer and neuroblastic layer.
TS19: telencephalon	Present	Regionally restricted		Expression is in the preplate and the ventricular zone of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex ventricular layer	Present
TS20: cortical preplate	Present
TS20: dorsal root ganglion	Present
TS20: vomeronasal organ	Present
TS20: neural retina	Present	Regionally restricted		Expression is in the retinal ganglion layer and neuroblastic layer.
TS20: telencephalon	Present	Regionally restricted		Expression is in the hippocampus.
TS20: brain nucleus	Present	Regionally restricted		Expression is in the dorsal lateral geniculate nucleus and ventral lateral geniculate nucleus.
TS20: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex intermediate zone	Present
TS21: cortical plate	Present
TS21: cortical subplate	Present
TS21: dorsal root ganglion	Present
TS21: retina ganglion cell layer	Present
TS21: vomeronasal organ	Present
TS21: neural retinal epithelium	Present	Regionally restricted		Expression is in the neuroblastic layer.
TS21: main olfactory bulb layer	Present	Regionally restricted		Expression is in the mitral layer and granule cell layer.
TS21: telencephalon	Present	Regionally restricted		Expression is in the hippocampus.
TS21: brain nucleus	Present	Regionally restricted		Expression is in the dorsal lateral geniculate nucleus and ventral lateral geniculate nucleus.
TS21: cerebral cortex marginal layer	Absent
TS21: cerebral cortex ventricular layer	Absent
TS21: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present
TS22: cortical plate	Present
TS22: hippocampus	Present
TS22: main olfactory bulb mitral cell layer	Present
TS22: retina ganglion cell layer	Present
TS22: vomeronasal organ	Present
TS22: neural retinal epithelium	Present	Regionally restricted		Expression is in the neuroblastic layer.
TS22: main olfactory bulb layer	Present	Regionally restricted		Expression is in the mitral layer and granule cell layer.
TS22: cerebral cortex marginal layer	Absent
TS22: cerebral cortex ventricular layer	Absent
TS22: cortical subplate	Absent
TS22: dorsal lateral geniculate nucleus	Absent
TS22: olfactory epithelium	Absent
TS22: ventral lateral geniculate nucleus	Absent

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present
TS24: cerebral cortex ventricular layer	Present
TS24: cortical plate	Present
TS24: dorsal lateral geniculate nucleus	Present
TS24: hippocampus	Present
TS24: main olfactory bulb mitral cell layer	Present
TS24: main olfactory bulb prospective granule cell layer	Present
TS24: retina ganglion cell layer	Present
TS24: retina nuclear layer	Present
TS24: ventral lateral geniculate nucleus	Present
TS24: vomeronasal organ	Present
TS24: cerebral cortex marginal layer	Absent
TS24: cortical subplate	Absent
TS24: olfactory epithelium	Absent

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present
TS27: cortical layer III	Present
TS27: cortical layer IV	Present
TS27: cortical layer V	Present
TS27: cortical layer VI	Present
TS27: dentate gyrus	Present
TS27: dorsal lateral geniculate nucleus	Present
TS27: hippocampus CA1	Present
TS27: hippocampus CA3	Present
TS27: main olfactory bulb granule cell layer	Present
TS27: main olfactory bulb mitral cell layer	Present
TS27: ventral lateral geniculate nucleus	Present
TS27: cerebral cortex ventricular zone	Absent
TS27: cortical layer I	Absent
TS27: olfactory epithelium	Absent
TS27: vomeronasal organ	Absent

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present
TS28: cortical layer III	Present
TS28: cortical layer IV	Present
TS28: cortical layer V	Present
TS28: cortical layer VI	Present
TS28: dentate gyrus	Present
TS28: hippocampus CA1	Present
TS28: hippocampus CA3	Present
TS28: ventral lateral geniculate nucleus	Present
TS28: ventral posterior lateral thalamic nucleus	Present
TS28: cerebral cortex ventricular zone	Absent
TS28: cortical layer I	Absent
TS28: dorsal lateral geniculate nucleus	Absent
TS28: ventrolateral thalamic nucleus	Absent