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Gene Expression Data
Assay Details
Assay
Reference: J:327901 Ye Q, et al., Understanding the development of oral epithelial organs through single cell transcriptomic analysis. Development. 2022 Aug 15;149(16):dev200539
Assay type: RNA in situ
MGI Accession ID: MGI:7615296
Gene symbol: Tfap2b
Gene name: transcription factor AP-2 beta
Probe: Mm-Tfap2b
Probe preparation: Antisense DNA
Visualized with: Fluorescence
Results
Specimen 7F: embryonic day 9.5 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - Shh (assay MGI:7615298).
Structure Level Pattern Image Note
TS15: 1st branchial arch mandibular component Ambiguous 7F' , 7F
Specimen 7G: embryonic day 10.0 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - Shh (assay MGI:7615298).
Structure Level Pattern Image Note
TS16: 1st branchial arch mandibular component ectoderm Present Regionally restricted 7G' , 7G Expression was detected in Pitx2-positive cells next to the developing heart.
Specimen 7H: embryonic day 10.5 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - Shh (assay MGI:7615298).
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Present Regionally restricted 7H , 7H' Expression expanded noticeably between E10.0 and E10.5. Boundary cells co-expressing Tfap2b with Pitx2 remained broadly distributed.
Specimen 7I: embryonic day 11.0 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - Shh (assay MGI:7615298).
Structure Level Pattern Image Note
TS18: 1st branchial arch mandibular component ectoderm Present 7I' , 7I
Specimen 7J: embryonic day 12.0 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - Shh (assay MGI:7615298).
Structure Level Pattern Image Note
TS20: mandibular process epithelium Present Regionally restricted 7J' , 7J Expression was detected in the zone anterior to the dental epithelium. Some overlap with Pitx2 expression was observed at the boundary of the anterior region and dental region.
Specimen S6D right: embryonic day 12.0 (more )
Note: Double labeled: green - Tfap2b; red - Zfp750 (assay MGI:7615304).
Structure Level Pattern Image Note
TS20: mandibular process epithelium Present Regionally restricted S6D' left , S6D right , S6D' right Expression was detected in the aboral epithelium.
TS20: periderm Present Regionally restricted S6D' right , S6D' left , S6D right Expression overlapped with Zfp750 staining in the periderm.
Specimen S10C left: embryonic day 9.5 (more )
Note: Double labeled: green - Tfap2b; red - Irx1 (assay MGI:7615072).
Structure Level Pattern Image Note
TS15: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10C left Expression was detected in the ventral-lateral arch.
Specimen S10C right: embryonic day 9.5 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - laminin.
Structure Level Pattern Image Note
TS15: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10C right Expression was detected in the ventral-lateral arch.
Specimen S10E: embryonic day 10.5 (more )
Note: Triple labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297); white - EdU.
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10E top right , S10E top left Expression was detected in aboral cells.
Specimen S10H: embryonic day 8.5 (more )
Note: Double labeled: green - Tfap2b; red - Pitx2 (assay MGI:7615297).
Structure Level Pattern Image Note
TS14: 1st branchial arch mandibular component ectoderm Absent S10H center
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory