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Gene Expression Data
Assay Details
Assay
Reference: J:327901 Ye Q, et al., Understanding the development of oral epithelial organs through single cell transcriptomic analysis. Development. 2022 Aug 15;149(16):dev200539
Assay type: RNA in situ
MGI Accession ID: MGI:7615297
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Probe: Mm-Pitx2 probe1
Probe preparation: Antisense DNA
Visualized with: Fluorescence
Results
Specimen 7F: embryonic day 9.5 (more )
Note: Triple labeled: red - Pitx2; white - Shh (assay MGI:7615298); green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS15: 1st branchial arch ectoderm Present Regionally restricted 7F' , 7F Expression was detected in the majority of ectodermal cells and border the Shh-positive endoderm.
Specimen 7G: embryonic day 10.0 (more )
Note: Triple labeled: red - Pitx2; white - Shh (assay MGI:7615298); green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS16: 1st branchial arch mandibular component ectoderm Present Regionally restricted 7G , 7G' Pitx2+ cells next to the developing heart now also express Tfap2b.
Specimen 7H: embryonic day 10.5 (more )
Note: Triple labeled: red - Pitx2; white - Shh (assay MGI:7615298); green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component ectoderm Present Regionally restricted 7H' , 7H Expression expanded noticeably between E10.0 and E10.5. Posteriorly, expression continued to adjoin the Shh+ endoderm.
TS17: oral region epithelium Present Regionally restricted 7H' , 7H Expression was located at the oral surface, but boundary cells co-expressing Tfap2b remain broadly distributed.
Specimen 7I: embryonic day 11.0 (more )
Note: Triple labeled: red - Pitx2; white - Shh (assay MGI:7615298); green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS18: oral region epithelium Present Regionally restricted 7I , 7I' Expression was detected in the dental epithelium.
Specimen 7J: embryonic day 12.0 (more )
Note: Triple labeled: red - Pitx2; white - Shh (assay MGI:7615298); green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS20: mandibular process epithelium Present Regionally restricted 7J , 7J' Expression was detected in the dental epithelium. Some overlap with Tfap2b expression was observed at the anterior boundary.
Specimen S9E E11.0: embryonic day 11.0 (more )
Note: Triple labeled: red - Pitx2; cyan - Rtl3 (assay MGI:7623178); green - Rxfp1 (assay MGI:7623179).
Structure Level Pattern Image Note
TS18: oral region epithelium Present Regionally restricted S9E E11.0 Expression was detected in dental epithelium.
Specimen S9E E11.5: embryonic day 11.5 (more )
Note: Triple labeled: red - Pitx2; cyan - Rtl3 (assay MGI:7623178); green - Rxfp1 (assay MGI:7623179).
Structure Level Pattern Image Note
TS19: mandibular process Present Regionally restricted S9E E11.5 Expression was detected in dental epithelium.
Specimen S9E E12.0: embryonic day 12.0 (more )
Note: Triple labeled: red - Pitx2; cyan - Rtl3 (assay MGI:7623178); green - Rxfp1 (assay MGI:7623179).
Structure Level Pattern Image Note
TS20: mandibular process epithelium Present Regionally restricted S9E E12.0 Expression was detected in dental epithelium.
Specimen S9E E12.5: embryonic day 12.5 (more )
Note: Triple labeled: red - Pitx2; cyan - Rtl3 (assay MGI:7623178); green - Rxfp1 (assay MGI:7623179).
Structure Level Pattern Image Note
TS20: mandibular process epithelium Present Regionally restricted S9E E12.5 Expression was detected in dental epithelium.
Specimen S10B: embryonic day 9.5 (more )
Note: Double labeled: red - Pitx2; white - laminin.
Structure Level Pattern Image Note
TS15: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10B right Expression was detected in the majority of ectodermal cells in medial mandible region.
Specimen S10C: embryonic day 9.5 (more )
Note: Triple labeled: red - Pitx2; green - Tfap2b (assay MGI:7615296); white - laminin.
Structure Level Pattern Image Note
TS15: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10C right Expression was detected in the majority of ectodermal cells in lateral mandible region.
Specimen S10D: embryonic day 10.5 (more )
Note: Double labeled: red - Pitx2; white - laminin.
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component Present Regionally restricted S10D right Expression was located at the oral surface.
Specimen S10E: embryonic day 10.5 (more )
Note: Triple labeled: red - Pitx2; green - Tfap2b (assay MGI:7615296); white - EdU.
Structure Level Pattern Image Note
TS17: 1st branchial arch mandibular component Present Regionally restricted S10E top left , S10E top right Expression was located at the oral surface.
Specimen S10H: embryonic day 8.5 (more )
Note: Double labeled: red - Pitx2; green - Tfap2b (assay MGI:7615296).
Structure Level Pattern Image Note
TS14: 1st branchial arch mandibular component ectoderm Present Regionally restricted S10H center Expression was detected in the dorsal two-thirds of the newly formed mandibular arch epithelium at E8.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory