Antibody:	anti-AP-2beta (#2509)
Detection system:	Secondary antibody coupled to Alexa Fluor 568

Structure	Level	Pattern	Image	Note
TS23: retina	Present		1A	Extensive co-localized immunostaining with Tfap2a was detected.

Structure	Cell Type	Level	Pattern	Image	Note
TS25: neural retina	retina horizontal cell	Present		2A	At this stage, 100% of Lhx1-positive cells in both the inner and outer retina were immunoreactive for Tfap2b.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neural retina	retina horizontal cell	Present		2B	At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neural retina	retina horizontal cell	Present		2C	At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: neural retina	retina horizontal cell	Present			At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: neural retina	retina horizontal cell	Strong	Regionally restricted	2D	Strong expression was detected in the future horizontal cell layer. Extensive overlap between Tfap2a and Tfap2b expression was observed (80% of immunopositive cells). In the outer retina, putative horizontal cells exhibiting strong Tfap2b signal were weakly stained for Tfap2a.

Structure	Level	Pattern	Image	Note
TS24: neural retina	Present	Regionally restricted	5A	Expression was detected both singly and co-labeled with Tfap2c. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Approximately 20% of the immunopositive population was double labeled.

Structure	Level	Pattern	Image	Note
TS27: neural retina	Present	Regionally restricted	5B	Expression was detected both singly and co-labeled with Tfap2c. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Approximately 20% of the immunopositive population was double labeled.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	5C	Expression was detected both singly and co-labeled with Tfap2c. The Tfap2c-positive population was smaller than the Tfap2b-positive population.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	5D	Expression was detected both singly and co-labeled with Tfap2c. The Tfap2c-positive population was smaller than the Tfap2b-positive population. Only 3.4% of the immunopositive population was double labeled in the mature retina.

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	S2B Wild-Type AP-2b	A staining domain was detected in central and peripheral retina.

Structure	Level	Pattern	Image	Note
TS26: retina	Absent		S2B Tcfap2a/b Mutant AP-2b