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Gene Expression Data
Assay Details
Assay
Reference: J:181601 Bassett EA, et al., Overlapping expression patterns and redundant roles for AP-2 transcription factors in the developing mammalian retina. Dev Dyn. 2012 Apr;241(4):814-29
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7578961
Gene symbol: Lhx1
Gene name: LIM homeobox protein 1
Antibody: anti-Lhx1/5 (4F2)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A: embryonic day 17.5 (more )
Note: Double labeled: green - Lhx1; red - Tfap2b (assay MGI:7578990).
Structure Cell Type Level Pattern Image Note
TS25: neural retina retina horizontal cell Present 2A At this stage, 100% of Lhx1-positive cells in both the inner and outer retina were immunoreactive for Tfap2b.
Specimen 2B: postnatal day 0 (more )
Note: Double labeled: green - Lhx1; red - Tfap2b (assay MGI:7578990).
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Present 2B At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.
Specimen 2C: postnatal day 7 (more )
Note: Double labeled: green - Lhx1; red - Tfap2b (assay MGI:7578990).
Structure Cell Type Level Pattern Image Note
TS28: neural retina retina horizontal cell Present 2C At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.
Specimen not shown: postnatal adult (more )
Structure Cell Type Level Pattern Image Note
TS28: neural retina retina horizontal cell Present At this stage, 100% of Lhx1-positive cells were immunoreactive for Tfap2b.
Specimen 7A: embryonic day 16.5 (more )
Structure Cell Type Level Pattern Image Note
TS24: neural retina retina horizontal cell Present Regionally restricted 7A Expression was detected in postmitotic horizontal cells.
Specimen 7B: embryonic day 16.5; Tfap2atm1Hsv/Tfap2atm1.1Hsv, Tfap2btm1Rbu/Tfap2btm1Rbu, Tg(Pax6-cre,GFP)2Pgr/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS24: neural retina retina horizontal cell Trace 7B The number of immunopositive cells in the double mutant retina was drastically reduced compared with controls.
Specimen 7C: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Present Regionally restricted 7C Expression was detected in postmitotic horizontal cells.
Specimen 7D: postnatal day 0; Tfap2atm1Hsv/Tfap2atm1.1Hsv, Tfap2btm1Rbu/Tfap2btm1Rbu, Tg(Pax6-cre,GFP)2Pgr/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS27: neural retina retina horizontal cell Trace Single cells 7D Expressing cells were rarely observed in the Tcfap2a/b-deficient regions of the retina.
Specimen not shown Control E17.5: embryonic day 17.5 (more )
Structure Cell Type Level Pattern Image Note
TS25: neural retina retina horizontal cell Present Regionally restricted Expression was detected in postmitotic horizontal cells.
Specimen not shown Tcfap2a/b E17.5: embryonic day 17.5; Tfap2atm1Hsv/Tfap2atm1.1Hsv, Tfap2btm1Rbu/Tfap2btm1Rbu, Tg(Pax6-cre,GFP)2Pgr/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS25: neural retina retina horizontal cell Trace Single cells Expressing cells were rarely observed in the Tcfap2a/b-deficient regions of the retina.
Specimen not shown Control E18.5: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: neural retina retina horizontal cell Present Regionally restricted Expression was detected in postmitotic horizontal cells.
Specimen not shown Tcfap2a/b E18.5: embryonic day 18.5 (more )
Structure Cell Type Level Pattern Image Note
TS26: neural retina retina horizontal cell Trace Single cells Expressing cells were rarely observed in the Tcfap2a/b-deficient regions of the retina.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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