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Gene Expression Data
Assay Details
Assay
Reference: J:321553 Neupane S, et al., O-fucosylation of thrombospondin type 1 repeats is essential for ECM remodeling and signaling during bone development. Matrix Biol. 2022 Feb 12;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7567583
Gene symbol: Hspa5
Gene name: heat shock protein 5
Antibody: anti-GRP78 BiP (ab21685)
Assay notes: Fixation was with 5% acetic acid in ethanol.
Results
Specimen 8A,C,D: embryonic day 14.5; Pofut2tm2.1Bch/Pofut2+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hspa5; red - Ccn2 (assay MGI:7567582).
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Regionally restricted 8D , 8C , 8D' , 8A Authors report perinuclear localization of expression.
Specimen 8E,G,H: embryonic day 14.5; Pofut2tm2.1Bch/Pofut2tm2.2Bch, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hspa5; red - Ccn2 (assay MGI:7567582).
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Regionally restricted 8H , 8E , 8G , 8H' Expression distinct from Ccn2 expression.
Specimen 8K,M,N: embryonic day 14.5; Pofut2tm2.1Bch/Pofut2+, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hspa5; red - Adamts17 (assay MGI:7567584).
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Regionally restricted 8N' , 8K , 8N , 8M Authors report perinuclear localization of expression.
Specimen 8O,Q,R: embryonic day 14.5; Pofut2tm2.1Bch/Pofut2tm2.2Bch, Tg(Prrx1-cre)1Cjt/0 (more )
Note: Conditional mutant. Double labeled: green - Hspa5; red - Adamts17 (assay MGI:7567584).
Structure Level Pattern Image Note
TS22: tibia cartilage condensation Present Regionally restricted 8R' , 8R , 8O , 8Q Expression distinct from Adamts17 expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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