Pofut2tm2.1Bch
Targeted Allele Detail
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Symbol: |
Pofut2tm2.1Bch |
Name: |
protein O-fucosyltransferase 2; targeted mutation 2.1, Bernadette C Holdener |
MGI ID: |
MGI:5811759 |
Synonyms: |
Pofut2-Floxed |
Gene: |
Pofut2 Location: Chr10:77095052-77105409 bp, + strand Genetic Position: Chr10, 39.72 cM
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Alliance: |
Pofut2tm2.1Bch page
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Germline Transmission: |
Earliest citation of germline transmission:
J:234992
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Parent Cell Line: |
Bruce 4 (ES Cell)
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Strain of Origin: |
B6.Cg-Thy1a
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Allele Type: |
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Targeted (Conditional ready, No functional change) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exons 3 and 4 of the targeted gene were replaced with a construct containing: loxP-FRT-polyA-neomycin (in reverse transcriptional orientation)-FRT-exons 3-9 of Pofut2 mini cDNA-polyA-loxP-Stop-mCherry-polyA. Flp-mediated recombination removed the FRT-flanked neo cassette. mCherry fluorescence is not detectable in this allele or in the allele generated by cre-mediated recombination.
(J:234992)
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Original: |
J:234992 Benz BA, et al., Genetic and biochemical evidence that gastrulation defects in Pofut2 mutants result from defects in ADAMTS9 secretion. Dev Biol. 2016 Aug 1;416(1):111-22 |
All: |
2 reference(s) |
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