Gene Expression Data
Assay Details
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Assay
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Reference:
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J:252112
Kim JJ, et al., Suppressor of fused controls cerebellar neuronal differentiation in a manner modulated by GLI3 repressor and Fgf15. Dev Dyn. 2018 Jan;247(1):156-169
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7567364
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Gene symbol:
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Fabp7
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Gene name:
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fatty acid binding protein 7, brain
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Antibody:
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anti-BLBP
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
4M:
embryonic day 13.5; Sufutm1Hui/Sufu +
(more  )
Note: Double labeled: green - Fabp7; red - Tubb3 (assay MGI:7565851).
Specimen
4M:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sufutm1Hui/Sufu+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fabp7; red - Tubb3 (assay MGI:7565851).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
4M
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Expression was detected in radial precursors. The spatial domains of Fabp7 (BLBP) and Tubb3 immunopositive cells could not be completely separated. A substantial area was Fabp7-positive but Tubb3-negative.
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| TS21: cerebellum ventricular layer |
Present |
Regionally restricted |
4M
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Expression was detected in radial precursors. The spatial domains of Fabp7 (BLBP) and Tubb3 immunopositive cells could not be completely separated. A substantial area was Fabp7-positive but Tubb3-negative.
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Specimen
4N:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sufutm1Hui/Sufutm1.1Hui
Tg(Fabp7-cre)1Htz/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Double labeled: green - Fabp7; red - Tubb3 (assay MGI:7565851).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
4N
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Expression was detected in radial precursors. The spatial domains of Fabp7 (BLBP) and Tubb3 immunopositive cells could not be completely separated. A substantial area was Fabp7-positive but Tubb3-negative. The area of the Fabp7 only immunopositive domain, relative to the total cerebellar area per section, was increased 1.8-fold in mutant cerebella at E13.5.
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| TS21: cerebellum ventricular layer |
Present |
Regionally restricted |
4N
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Expression was detected in radial precursors. The spatial domains of Fabp7 (BLBP) and Tubb3 immunopositive cells could not be completely separated. A substantial area was Fabp7-positive but Tubb3-negative. The area of the Fabp7 only immunopositive domain, relative to the total cerebellar area per section, was increased 1.8-fold in mutant cerebella at E13.5.
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Specimen
4O:
embryonic day 13.5; Sufutm1Hui/Sufu +
(more )
Specimen
4O:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sufutm1Hui/Sufu+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4P:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sufutm1Hui/Sufutm1.1Hui
Tg(Fabp7-cre)1Htz/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
Regionally restricted |
4P
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Expression was upregulated in radial precursors in the mutant compared with control.
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| TS21: cerebellum ventricular layer |
Present |
Regionally restricted |
4P
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Expression was upregulated in radial precursors in the mutant compared with control.
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Specimen
7K:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Gli3tm1Urt/?
Sufutm1Hui/Sufutm1.1Hui
Tg(Fabp7-cre)1Htz/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Double labeled: green - Fabp7; red - Tubb3 (assay MGI:7565851).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebellum |
Present |
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7K
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Expression domain in SuFu<-/loxP>;Blbp-Cre;Gli3<delta699> cerebella was comparable to control mice.
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Specimen
7L:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Sufutm1Hui/Sufutm1.1Hui
Tg(Fabp7-cre)1Htz/0
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant. Double labeled: green - Fabp7; red - Tubb3 (assay MGI:7565851).
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Analysis Tools