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Gene Expression Data
Assay Details
Assay
Reference: J:233705 Zhao H, et al., Deletions of Retinoblastoma 1 (Rb1) and Its Repressing Target S Phase Kinase-associated protein 2 (Skp2) Are Synthetic Lethal in Mouse Embryogenesis. J Biol Chem. 2016 May 6;291(19):10201-9
Assay type: RT-PCR
MGI Accession ID: MGI:7562451
Gene symbol: Ccna2
Gene name: cyclin A2
Probe: Cyclin A pF, Cyclin A pR
Assay notes: Quantitative RT-PCR. Relative expression was normalized to Gapdh.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
Rb1lox/lox E13.5 TS21: brain Present 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3TyjNot Specified
Nes-Cre;Rb1lox/lox E13.5 TS21: brain Present (a) 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Rb1lox/lox;Skp2-/- E13.5 TS21: brain Present 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1KinNot Specified
Nes-Cre;Rb1lox/lox;Skp2-/- E13.5 TS21: brain Present (b) 2.5 µg; total RNA involves: 129 * C57BL/6J * FVB Rb1tm3Tyj/Rb1tm3Tyj, Skp2tm1Kin/Skp2tm1Kin, Tg(Nes-cre)1Kln/0Not Specified Conditional mutant.
Notes:
(a) Increased expression compared with the Rb1<lox/lox> control.
(b) Skp2-/- did not correct the increased expression but instead increased the expression further.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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