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Gene Expression Data
Assay Details
Assay
Reference: J:293091 Li Z, et al., Neural progenitor cells mediated by H2A.Z.2 regulate microglial development via Cxcl14 in the embryonic brain. Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24122-24132
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7544053
Gene symbol: Aif1
Gene name: allograft inflammatory factor 1
Results
Specimen S1 Iba1: embryonic day 16.5 (more )
Note: Double labeled: red - Aif1; green - Cd68 (assay MGI:7544051).
Structure Level Pattern Image Note
TS24: cerebral cortex Present S1A (right) , S1A (right inset lower) , S1A (right inset middle) Expression in virtually all Cd68-expressing cells.
Specimen 1A E13.5: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: cerebral cortex microglial cell Present 1A E13.5
Specimen E13.5 not shown: embryonic day 13.5 (more )
Note: Data presented in Figs. 1B and 1C.
Structure Level Pattern Image Note
TS21: pallium Weak
Specimen 1A E16.5: embryonic day 16.5 (more )
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present 1A E16.5
Specimen E16.5 not shown: embryonic day 16.5 (more )
Note: Data presented in Figs. 1B-E.
Structure Level Pattern Image Note
TS24: pallium Present Expression significantly increased upon neurogenesis in cortical and subcortical areas
TS24: subpallium Present Expression significantly increased upon neurogenesis in cortical and subcortical areas
TS24: cerebral cortex subventricular zone Strong Expression preferentially clustered in ventricular/subventricular zone.
TS24: cerebral cortex ventricular layer Strong Expression preferentially clustered in ventricular/subventricular zone.
Specimen 1A P0: postnatal day 0 (more )
Structure Cell Type Level Pattern Image Note
TS27: cerebral cortex microglial cell Present 1A P0
Specimen P0 not shown: postnatal day 0 (more )
Note: Data presented in Figs. 1B and 1C.
Structure Level Pattern Image Note
TS27: pallium Strong Expression significantly increased upon neurogenesis in cortical and subcortical areas
TS27: subpallium Strong Expression significantly increased upon neurogenesis in cortical and subcortical areas
Specimen 2F fl/fl: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko (more )
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex subventricular zone microglial cell Present 2F fl/fl (merged) , 2F fl/fl Iba1
TS24: cerebral cortex ventricular layer microglial cell Present 2F fl/fl (merged) , 2F fl/fl Iba1
Specimen 2F cKO: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant.
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex subventricular zone microglial cell Strong 2F cKO (merged) , 2F cKO Iba1 Increase in number of microglia in ventricular/subventricular zone compared to control.
TS24: cerebral cortex ventricular layer microglial cell Strong 2F cKO (merged) , 2F cKO Iba1 Increase in number of microglia in ventricular/subventricular zone compared to control.
Specimen 2H fl/fl: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko (more )
Note: Double labeled: green - Aif1; red - Sox2 (assay MGI:7544058).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present 2H fl/fl (right lower) , 2H fl/fl (left) , 2H fl/fl (right upper) , 2H fl/fl (center)
Specimen 2H cKO: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: green - Aif1; red - Sox2 (assay MGI:7544058).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex subventricular zone microglial cell Present 2H cKO (left) , 2H cKO (right lower) , 2H cKO (right upper) , 2H cKO (center) Contact between microglia and neural progenitor cells in ventricular/subventricular zone was enhancedcompared to control.
TS24: cerebral cortex ventricular layer microglial cell Present 2H cKO (left) , 2H cKO (right lower) , 2H cKO (right upper) , 2H cKO (center) Contact between microglia and neural progenitor cells in ventricular/subventricular zone was enhancedcompared to control.
Specimen S6A fl/fl: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko (more )
Note: Double labeled: red - Aif1; green - Arg1 (assay MGI:7544067).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present S6A fl/fl Iba1 , S6A fl/fl (merged)
Specimen S6A cKO: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Arg1 (assay MGI:7544067).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present S6A cKO (merged) , S6A cKO Iba1
Specimen S6C fl/fl: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko (more )
Note: Double labeled: red - Aif1; green - Nos2 (assay MGI:7544068).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present S6C fl/fl (merged) , S6C fl/fl Iba1
Specimen S6C cKO: embryonic day 16.5; H2az2tm1.1Hko/H2az2tm1.1Hko, Tg(Nes-cre)1Kln/0 (more )
Note: Conditional mutant. Double labeled: red - Aif1; green - Nos2 (assay MGI:7544068).
Structure Cell Type Level Pattern Image Note
TS24: cerebral cortex microglial cell Present S6C cKO Iba1 , S6C cKO (merged)
Specimen 3J Iba1: embryonic day 16.5 (more )
Note: Double labeled: red - Aif1; green - Cxcr4 (assay MGI:7544062).
Structure Level Pattern Image Note
TS24: cerebral cortex Present 3J Iba , 3J (merged)
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
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