About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520518
Gene symbol: Gorasp1
Gene name: golgi reassembly stacking protein 1
Antibody: anti-GRASP65 [EPR12439] (ab30315)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
Results
Specimen 3B Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls.
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present Regionally restricted 3B Control , 3B Control +DAPI Golgi apparatus as radially aligned short segments in ventricular zone.
Specimen 3B DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3B DKO +DAPI , 3B DKO Pattern of expression predominantly punctate and in perinuclear location.
Specimen S3A Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls.
Structure Level Pattern Image Note
TS20: neocortex Present Punctate S3A Control , S3A Control +DAPI Punctate expression in upper neocortical layers.
TS20: cerebral cortex ventricular layer Present Regionally restricted S3A Control +DAPI , S3A Control In lower layers, expression in radially aligned short segments.
Specimen S3A DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre.
Structure Level Pattern Image Note
TS20: neocortex Present Punctate S3A DKO , S3A DKO +DAPI Punctate expression in upper neocortical layers
TS20: cerebral cortex ventricular layer Present Punctate S3A DKO , S3A DKO +DAPI Perinuclear punctate expression in deep neocortical layers
Specimen S3B Grasp65 Control: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: green - Gorasp1; red - Nes (assay MGI:7520517).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted S3B Control Grasp65 , S3B Control Merge Similar radial alignment and Goligi distribution profiles in control and DKO embryos at E11.5.
Specimen S3B Grasp65 DKO: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Gorasp1; red - Nes (assay MGI:7520517).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted S3B DKO Merge , S3B DKO Grasp65 Similar radial alignment and Goligi distribution profiles in control and DKO embryos at E11.5.
Specimen S3G Grasp65: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Gorasp1; red - F-actin.
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present S3G
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory