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Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520518
Gene symbol: Gorasp1
Gene name: golgi reassembly stacking protein 1
Antibody: anti-GRASP65 [EPR12439] (ab30315)
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Cy2
Results
Specimen 3B Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls.
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present Regionally restricted 3B Control , 3B Control +DAPI Golgi apparatus as radially aligned short segments in ventricular zone.
Specimen 3B DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre.
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3B DKO +DAPI , 3B DKO Pattern of expression predominantly punctate and in perinuclear location.
Specimen S3A Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls.
Structure Level Pattern Image Note
TS20: neocortex Present Punctate S3A Control , S3A Control +DAPI Punctate expression in upper neocortical layers.
TS20: cerebral cortex ventricular layer Present Regionally restricted S3A Control +DAPI , S3A Control In lower layers, expression in radially aligned short segments.
Specimen S3A DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre.
Structure Level Pattern Image Note
TS20: neocortex Present Punctate S3A DKO , S3A DKO +DAPI Punctate expression in upper neocortical layers
TS20: cerebral cortex ventricular layer Present Punctate S3A DKO , S3A DKO +DAPI Perinuclear punctate expression in deep neocortical layers
Specimen S3B Grasp65 Control: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: green - Gorasp1; red - Nes (assay MGI:7520517).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted S3B Control Grasp65 , S3B Control Merge Similar radial alignment and Goligi distribution profiles in control and DKO embryos at E11.5.
Specimen S3B Grasp65 DKO: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Gorasp1; red - Nes (assay MGI:7520517).
Structure Level Pattern Image Note
TS19: telencephalon Present Regionally restricted S3B DKO Merge , S3B DKO Grasp65 Similar radial alignment and Goligi distribution profiles in control and DKO embryos at E11.5.
Specimen S3G Grasp65: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: green - Gorasp1; red - F-actin.
Structure Level Pattern Image Note
TS20: cerebral cortex ventricular layer Present S3G
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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