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Gene Expression Data
Assay Details
Assay
Reference: J:263975 Xie Z, et al., A Golgi Lipid Signaling Pathway Controls Apical Golgi Distribution and Cell Polarity during Neurogenesis. Dev Cell. 2018 Mar 26;44(6):725-740.e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7520517
Gene symbol: Nes
Gene name: nestin
Antibody: anti-Nestin (4760)
Detection system: Biotinylated secondary antibody/[Strept]avidin
Results
Specimen 3A Nestin Control: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Nes; green - Casp3 (assay MGI:7520514).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A Control Merge , 3A Control Nestin Expression in apical and basal process of neural stem cells. Nestin fibers displayed a general radial orientation.
Specimen 3A Nestin DKO: embryonic day 12.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Nes; green - Casp3 (assay MGI:7520514).
Structure Level Pattern Image Note
TS20: neocortex Present Regionally restricted 3A DKO Merge , 3A DKO Nestin Radial orientation of nestin fibers not discernible throughout neocortex.
Specimen S3B Nestin Control: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/?, Pitpnbtm2.1Vab/? (more )
Note: Conditional mutant. Littermate embryos with at least one non-floxed allele of Pitpna or Pitpnb (i.e. Pitpnafl/+ Pitpnbfl/fl Emx1Cre/+, Pitpnafl/+ Pitpnbfl/fl Emx1Cre/Cre, Pitpnafl/fl Pitpnbfl/+ Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/+ Emx1Cre/Cre) were used as controls. Double labeled: red - Nes; green - Gorasp1 (assay MGI:7520518).
Structure Level Pattern Image Note
TS19: telencephalon Present S3B Control Nestin , S3B Control Merge
Specimen S3B Nestin DKO: embryonic day 11.5; Emx1tm1(cre)Krj/?, Pitpnatm2.1Vab/Pitpnatm2.1Vab, Pitpnbtm2.1Vab/Pitpnbtm2.1Vab (more )
Note: Conditional mutant. Tissue-specific double knockout progeny (designated DKO) were either Pitpnafl/fl Pitpnbfl/fl Emx1Cre/+ or Pitpnafl/fl Pitpnbfl/fl Emx1Cre/Cre. Double labeled: red - Nes; green - Gorasp1 (assay MGI:7520518).
Structure Level Pattern Image Note
TS19: telencephalon Present S3B DKO Merge , S3B DKO Nestin Authors report no defects in neocortex compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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