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Gene Expression Data
Assay Details
Assay
Reference: J:234197 Wong BH, et al., Mfsd2a Is a Transporter for the Essential omega-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem. 2016 May 13;291(20):10501-14
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7507598
Gene symbol: Mfsd2a
Gene name: MFSD2 lysolipid transporter A, lysophospholipid
Antibody: anti-MFSD2A
Results
Specimen 1A WT Mfsd2a: postnatal day 8 (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Present Regionally restricted 1A WT Mfsd2a , 1A WT Merge
Specimen 1A WT Vascular Sprout Mfsd2a: postnatal day 8 (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Present Regionally restricted 1A WT Vascular Sprout Expression only in mature capillaries.
Specimen 1A WT Tip Cells Mfsd2a: postnatal day 8 (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Present Regionally restricted 1A WT Tip Cells Expression absent in tip cells at leading edge of vasculature.
Specimen 1A KO Mfsd2a: postnatal day 8; Mfsd2atm1Dls/Mfsd2atm1Dls (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Level Pattern Image Note
TS28: retina Absent 1A KO Merge , 1A KO Mfsd2a
Specimen 1A KO Vascular Sprout Mfsd2a: postnatal day 8; Mfsd2atm1Dls/Mfsd2atm1Dls (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Level Pattern Image Note
TS28: retina blood vessel Absent 1A KO Vascular Sprout
Specimen 1A KO Tip Cells Mfsd2a: postnatal day 8; Mfsd2atm1Dls/Mfsd2atm1Dls (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Level Pattern Image Note
TS28: retina blood vessel Absent 1A KO Tip Cells
Specimen 1B WT Mfsd2a: postnatal day 14 (more )
Note: Double labeled: red - Mfsd2a; green - isolectin.
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Present Regionally restricted 1B WT Merge , 1B WT Mfsd2a Expression in capillaries.
TS28: pigmented retinal epithelium Present 1B WT Merge , 1B WT Mfsd2a
Specimen 1B KO Mfsd2a: postnatal day 14; Mfsd2atm1Dls/Mfsd2atm1Dls (more )
Note: Double labeled: red - Mfsd2a; green - isolectin.
Structure Level Pattern Image Note
TS28: retina blood vessel Absent 1B KO Merge , 1B KO Mfsd2a
TS28: pigmented retinal epithelium Absent 1B KO Mfsd2a , 1B KO Merge
Specimen 1C WT Mfsd2a: postnatal day 14 (more )
Note: Double labeled: red - Mfsd2a; green - Ezr (assay MGI:7507602).
Structure Level Pattern Image Note
TS28: pigmented retinal epithelium Present Regionally restricted 1C WT Merge , 1C WT Mfsd2a Expression in apical and basolateral membranes in retina pigmented epithelium.
Specimen 1C KO Mfsd2a: postnatal day 14; Mfsd2atm1Dls/Mfsd2atm1Dls (more )
Note: Double labeled: red - Mfsd2a; green - Ezr (assay MGI:7507602).
Structure Level Pattern Image Note
TS28: retina blood vessel Absent 1C KO Merge , 1C KO Mfsd2a
TS28: pigmented retinal epithelium Absent 1C KO Merge , 1C KO Mfsd2a
Specimen 8A LL Mfsd2a: postnatal day 9; Mfsd2atm1c(KOMP)Wtsi/Mfsd2atm1c(KOMP)Wtsi (more )
Note: Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Present Regionally restricted 8A LL Mfsda , 8A LL Merge Expression in capillaries.
Specimen 8A LLTie2Cre Mfsd2a: postnatal day 9; Mfsd2atm1c(KOMP)Wtsi/Mfsd2atm1c(KOMP)Wtsi, Tg(Tek-cre)1Ywa/0 (more )
Note: Conditional mutant. Double labeled: green - Mfsd2a; red - Pecam1 (assay MGI:7507597).
Structure Cell Type Level Pattern Image Note
TS28: retina blood vessel retinal blood vessel endothelial cell Absent 8A LLTie2Cre Mfsd2a , 8A LLTie2Cre Merge Expression deleted in retinal capillaries.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
MGI 6.23 		The Jackson Laboratory