Mfsd2a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mfsd2a^{tm1c(KOMP)Wtsi}
• Name: MFSD2 lysolipid transporter A, lysophospholipid; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5805836
• Gene: Mfsd2a Location: Chr4:122840643-122854981 bp, - strand Genetic Position: Chr4, 57.34 cM, cytoband D1
• Alliance: Mfsd2a^{tm1c(KOMP)Wtsi} page
• IMPC: Mfsd2a gene page

Mutation origin

• Mutant Cell Line: EPD0853_5_E03
• Germline Transmission: Earliest citation of germline transmission: J:234197
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 122850309 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 3 at position 122850988. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice, removing the lacZ sequences and neomycin selection cassette. Exon 3 remains flanked by loxP sites. (J:234197)

Expression

In Mice Carrying this Mutation:	9 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mfsd2a Mutation: 40 strains or lines available

References

• Original: J:234197 Wong BH, et al., Mfsd2a Is a Transporter for the Essential omega-3 Fatty Acid Docosahexaenoic Acid (DHA) in Eye and Is Important for Photoreceptor Cell Development. J Biol Chem. 2016 May 13;291(20):10501-14
• All: 6 reference(s)