Gene Expression Data
Assay Details
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Assay
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Reference:
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J:333055
Basta LP, et al., Celsr1 and Celsr2 exhibit distinct adhesive interactions and contributions to planar cell polarity. Front Cell Dev Biol. 2022;10:1064907
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:7468940
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Gene symbol:
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Vangl2
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Gene name:
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VANGL planar cell polarity 2
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Results
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Specimen
3B':
embryonic day 15.5
(more  )
Note: Back skin
Specimen
3B':
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Back skin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: epidermis basal layer |
basal cell of epidermis |
Present |
Regionally restricted |
3B' top
,
3B' bottom
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Expression was detected in the basal layer of the interfollicular epidermis enriched along anterior-posterior (A-P) junctions and depleted from M-L junctions.
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Specimen
3D':
(close )
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Genetic Background:
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B6.Cg-Celsr1em1Ddev
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Mutant Allele(s):
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Celsr1em1Ddev/Celsr1em1Ddev
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Back skin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: epidermis basal layer |
basal cell of epidermis |
Present |
Regionally restricted |
3D' top
,
3D' bottom
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Expression was more uniform around the periphery of basal cells compared to controls.
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Specimen
3F':
(close )
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Genetic Background:
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B6.Cg-Celsr2em1Ddev
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Mutant Allele(s):
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Celsr2em1Ddev/Celsr2em1Ddev
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Back skin
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| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: epidermis basal layer |
basal cell of epidermis |
Present |
Regionally restricted |
3F' top
,
3F' bottom
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Asymmetry was mostly unaffected in the Celsr2-/- mutant. Staining localization appeared less sharply concentrated at junctions.
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Specimen
3H':
(close )
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS23: epidermis basal layer |
basal cell of epidermis |
Present |
Regionally restricted |
3H' top
,
3H' bottom
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Staining asymmetries were more severely reduced in Celsr1-/-;Celsr2-/- double mutants compared to Celsr1-/- single mutants. However, there was not an appreciable reduction in membrane recruitment of Vangl2.
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Analysis Tools