Antibody:	Anti-Sox9 (AB5535)
Detection system:	Secondary antibody coupled to Alexa Fluor 555

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Regionally restricted	2A top , 2A lower right , 2A lower left	Expression was detected in a population of cells on the posterior of polarized hair follicles. The cells are distinct from the Cdh3 labeled population.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Regionally restricted	2B lower left , 2B lower right , 2B top	Over 90% of the hair follicles were unpolarized and displayed vertically-oriented growth. The Sox9 immunostaining was observed as a ring of cells surrounding a central cluster of Cdh3 immunostained cells (described as a bicycle wheel like ring of staining). Few follicles displayed asymmetry and were oriented randomly.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Regionally restricted	2C top , 2C lower right , 2C lower left	Expression was indistinguishable from wild type. Proper Anterior-posterior orientation was noted.

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Regionally restricted	2D lower left , 2D lower right , 2D top	Expression was indistinguishable from Celsr1-/- immunostaining.