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Gene Expression Data
Assay Details
Assay
Reference: J:146199 Barrionuevo F, et al., Testis cord differentiation after the sex determination stage is independent of Sox9 but fails in the combined absence of Sox9 and Sox8. Dev Biol. 2009 Mar 15;327(2):301-12
Assay type: RT-PCR
MGI Accession ID: MGI:7464365
Gene symbol: Wt1
Gene name: WT1 transcription factor
Probe: Wt1-pF1, Wt1-pR1
Probe preparation: labelled with SYBR green
Assay notes: Two primer sets were described, but the one used in this assay was not detailed. Wt1-pF2, Wt1-pR2 MGI:7464288 may have been used in this assay.
Results
Image: 5Aa ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
XY Control E15.5 TS23: testis Present Not Specified; total RNA involves: C57BL/6 Male
XY Sox9delta/delta E15.5 TS23: testis Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * C57BL/6 * SJL Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/0Male Conditional mutant.
XY Sox8-/- E15.5 TS23: testis Present (a) Not Specified; total RNA involves: 129S1/Sv * 129X1/SvJ Sox8tm1Weg/Sox8tm1WegMale
XY Sox9delta/delta;Sox8-/- E15.5 TS23: testis Present (a) Not Specified; total RNA involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL Sox8tm1Weg/Sox8tm1Weg, Sox9tm1Gsr/Sox9tm1Gsr, Plekha5Tg(AMH-cre)1Flor/Plekha5+Male Conditional mutant.
Notes:
(a) No difference compared to XY control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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