Probe:	Barhl2 cDNA
Probe preparation:	Antisense labelled with fluorescein RNA
Visualized with:	Fluorescence

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	4B , 4C	Expressed in a narrower region, extending from the floorplate to the roofplate, whose alar domain overlaps a central portion of the diencephalic domain of Pax6 expression.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	4E , 4F	Expressed in the zona limitans intrathalamica (Pax6-) as a thin domain and the thalamus, but not in the prethalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4H , 4I	Expressed in zona limitans intrathalamica (ZLI), the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone and the hypothalamus. Expression remained low or absent in the narrow strip of progenitor cells (pTh-R) caudal to ZLI.

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present		4L , 4K

Structure	Level	Pattern	Image	Note
TS21: zona limitans intrathalamica	Present		4O , 4N

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	5D , 5B , 5A	Expressed primarily in the cytoplasm in the presumptive zona limitans intrathalamica, and also in the region of prethalamic neuroepithelium directly rostral to it. Many cells in this region co-expressed Pax6.

Structure	Level	Pattern	Image	Note
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	6A	Co-expressed with Pax6 in the pretectum.
TS20: thalamus	Present		6H , 6E , 6I , 6A , 6D	Close to the pretectum, almost all cells co-expressed with Pax6.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	6L , 6M	Expressed in the eminentia thalami (Pax6-).

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Present	Regionally restricted	7B , 7C	Expressed in the thalamus, with levels increasing towards the zona limitans intrathalamica. No expression in the prethalamus.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	7F , 7E	Expressed in the thalamus, with levels increasing towards the zona limitans intrathalamica.
TS19: prethalamus	Absent		7E , 7F

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Graded	7I , 7H	Counter-gradient expression of that of Pax6, with levels increasing towards the zona limitans intrathalamica.
TS20: prethalamus	Absent		7H , 7I

Structure	Level	Pattern	Image	Note
TS21: thalamus ventricular layer	Present		7K , 7L	There was no longer any obvious gradient. Relatively strongly expressed in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present	Regionally restricted	11A , 11C	Expressed in the differentiating but not the proliferating zone of the eminentia thalami.
TS19: telencephalon	Present	Regionally restricted	11A , 11C	Not expressed in dorsal telencephalon.
TS19: diencephalon	Present	Regionally restricted	11A , 11C	Expressed in zona limitans intrathalamica (ZLI), and the region of progenitor cells (pTh-C domain). Not expressed in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS19: prethalamus	Absent		11A , 11C

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	11F , 11D	Expressed in the differentiating but not the proliferating zone of the eminentia thalami.
TS20: pallium	Absent		11D , 11F	dorsal telencephalon
TS20: prethalamus	Absent		11D , 11F	dorsal telencephalon
TS20: thalamus	Present	Regionally restricted	11D , 11F	Expressed in the region of progenitor cells (pTh-C domain). Not expressed in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS20: zona limitans intrathalamica	Present		11F , 11D

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	11I , 11G	Expressed in the differentiating but not the proliferating zone of the eminentia thalami.
TS21: pallium	Absent		11I , 11G	dorsal telencephalon
TS21: prethalamus	Absent		11I , 11G	dorsal telencephalon
TS21: zona limitans intrathalamica	Present		11I , 11G
TS21: thalamus	Present	Regionally restricted	11G , 11I	Expressed in the region of progenitor cells (pTh-C domain). Not expressed in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.

Structure	Level	Pattern	Image	Note
TS20: thalamus	Present	Regionally restricted	11J , 11L	Expressed in the region of progenitor cells (pTh-C domain). Not expressed in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica.
TS20: zona limitans intrathalamica	Present		11L , 11J

Structure	Level	Pattern	Image	Note
TS20: zona limitans intrathalamica	Present	Regionally restricted	11O , 11M	Expression was slightly narrower than that of Shh within the ZLI and was centered caudally.