Gene Expression Data
Assay Details
Assay
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Reference:
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J:309436
Parish EV, et al., Expression of Barhl2 and its relationship with Pax6 expression in the forebrain of the mouse embryo. BMC Neurosci. 2016 Nov 25;17(1):76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:7461464
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 cDNA16
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Probe preparation:
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Antisense labelled with fluorescein RNA
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Visualized with:
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Fluorescence
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Results
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Specimen
4A/C:
embryonic day 9.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
4A/C:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
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Specimen
4D/F:
embryonic day 10.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
4D/F:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
|
Specimen
4G/I:
embryonic day 11.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
4G/I:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: diencephalon |
Present |
Regionally restricted |
4I
,
4G
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Expressed in the region of progenitor cells (pTh-C domain) in the thalamic ventricular zone. Expression remained low or absent in the narrow strip of progenitor cells (pTh-R) caudal to the zona limitans intrathalamica. No expression in zona limitans intrathalamica.
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TS19: prethalamus |
Present |
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4I
,
4G
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TS19: forebrain-midbrain boundary region |
Present |
Regionally restricted |
4I
,
4G
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Expressed in the pretectum.
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Specimen
4J/L:
embryonic day 12.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
4J/L:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
|
Specimen
4M/O:
embryonic day 13.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
4M/O:
(close )
Genetic Background:
|
CD-1
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
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Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
|
Specimen
5A/B/D:
embryonic day 9.5
(more )
Note: Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
Specimen
5A/B/D:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Note:
|
Double labeled: green - Pax6; magenta - Barhl2 (assay MGI:7461456).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
5D
,
5B
,
5A
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Expressed in the nucleus in the presumptive zona limitans intrathalamica, and also in the region of prethalamic neuroepithelium (all cells) directly rostral to it. Many cells in this region co-expressed Barhl2.
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