Plvap In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:328290 Nakagawa T, et al., A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis. Cell Rep. 2021 Oct 19;37(3):109875
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:7461237
Gene symbol:	Plvap
Gene name:	plasmalemma vesicle associated protein

Reporter:	Cre
Antibody:	Anti-Cre (D7L7L; 15036)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Results

S3B CreER ©

S3B MERGE ©

Specimen S3B: postnatal adult; Plvap^{tm1.1(cre/ERT2)Tng}/Plvap⁺ (more

)
Note: Triple labeled: green - Plvap reporter; magenta - Plvap protein (assay MGI:7461459); cyan - Gfra1 (assay MGI:7461477).

Structure	Level	Pattern	Image	Note
TS28: spermatogonium	Present	Regionally restricted	S3B CreER , S3B MERGE
TS28: testis	Present	Regionally restricted	S3B CreER , S3B MERGE
TS28: spermatid	Present		S3B MERGE , S3B CreER	This is ectopic expression.

S3D CreER ©

S3D MERGE ©

Specimen S3D: postnatal adult; Plvap^{tm1.1(cre/ERT2)Tng}/Plvap⁺ (more

)
Note: Triple labeled: green - Plvap reporter; magenta - Plvap protein (assay MGI:7461459); cyan - Gfra1 (assay MGI:7461477).

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present	Regionally restricted	S3D CreER , S3D MERGE
TS28: spermatogonium	Present	Regionally restricted	S3D CreER , S3D MERGE

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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