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Gene Expression Data
Assay Details
Assay
Reference: J:328290 Nakagawa T, et al., A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis. Cell Rep. 2021 Oct 19;37(3):109875
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7461459
Gene symbol: Plvap
Gene name: plasmalemma vesicle associated protein
Antibody: clone MECA-32
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 1E: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted 1E Expression is seen in the least differentiated spermatogonia.
TS28: testis Present Regionally restricted 1E Expression is seen in the least differentiated spermatogonia.
Specimen 1F: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1F Expression is seen in the least differentiated spermatogonia. These spermatogonia are scattered sparsely on the basement membrane of seminiferous tubules, with biased localization to the interstitium.
TS28: spermatogonium Present Regionally restricted 1F Expression is seen in the least differentiated spermatogonia. These spermatogonia are scattered sparsely on the basement membrane of seminiferous tubules, with biased localization to the interstitium.
Specimen 1G: postnatal adult (more )
Note: Triple labeled: magenta - Plvap; cyan - Gfra1 (assay MGI:7461477); green - Sox3 (assay MGI:7461480).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted 1G PLVAP , 1G MERGE Expression is seen in the least differentiated spermatogonia.
TS28: spermatogonium Present Regionally restricted 1G PLVAP , 1G MERGE Expression is seen in the least differentiated spermatogonia.
Specimen S2A - upper: postnatal adult (more )
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S2A upper Expression is seen in the least differentiated spermatogonia. These spermatogonia are located on basement membrane, in regions facing the interstitium.
TS28: testis Present Regionally restricted S2A upper Expression is seen in the least differentiated spermatogonia. These spermatogonia are located on basement membrane, in regions facing the interstitium.
Specimen S2A - lower: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S2A lower Expression is seen in the least differentiated spermatogonia. These spermatogonia are located on basement membrane, in regions facing the adjacent seminiferous tubule.
TS28: testis Present Regionally restricted S2A lower Expression is seen in the least differentiated spermatogonia. These spermatogonia are located on basement membrane, in regions facing the adjacent seminiferous tubule.
Specimen S2D: postnatal adult; Ttm1(cre/ERT2)Hssk/T+ (more )
Note: Multi-labeled: magenta - Plvap; green - T (assay MGI:7461503); yellow - Pdx1 (assay MGI:7461502); cyan - Gfra1 (with Pdx1; assay MGI:7461477).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S2D Merge , S2D PLVAP Expression is seen in the least differentiated spermatogonia.
TS28: spermatogonium Present Regionally restricted S2D Merge , S2D PLVAP Expression is seen in the least differentiated spermatogonia.
Specimen S3B: postnatal adult; Plvaptm1.1(cre/ERT2)Tng/Plvap+ (more )
Note: Triple labeled: magenta - Plvap protein; green - Plvap reporter (assay MGI:7461237); cyan - Gfra1 (assay MGI:7461477).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S3B PLVAP , S3B MERGE
TS28: testis Present Regionally restricted S3B PLVAP , S3B MERGE
Specimen S3C: postnatal adult (more )
Note: Wild type. Double labeled: magenta - Plvap; cyan - Gfra1 (assay MGI:7461477).
Structure Level Pattern Image Note
TS28: spermatogonium Present Regionally restricted S3C MERGE , S3C PLVAP
TS28: testis Present Regionally restricted S3C MERGE , S3C PLVAP
Specimen S3D: postnatal adult; Plvaptm1.1(cre/ERT2)Tng/Plvap+ (more )
Note: Triple labeled: magenta - Plvap protein; green - Plvap reporter (assay MGI:7461237); cyan - Gfra1 (assay MGI:7461477).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S3D PLVAP , S3D MERGE
TS28: spermatogonium Present Regionally restricted S3D PLVAP , S3D MERGE
Specimen S3E: postnatal adult (more )
Note: Wild type. Double labeled: magenta - Plvap; cyan - Gfra1 (assay MGI:7461477).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Regionally restricted S3E MERGE , S3E PLVAP
TS28: spermatogonium Present Regionally restricted S3E MERGE , S3E PLVAP
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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