Probe:	Lmo4 probe5
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS25: central nervous system	Present		1B

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		2A	Expression in all three major embryonic layers.
TS11: embryo endoderm	Present		2A	Expression in all three major embryonic layers.
TS11: embryo mesoderm	Present		2A	Expression in all three major embryonic layers.

Structure	Level	Pattern	Image	Note
TS16: neural ectoderm	Present	Widespread	2B	Widespread expression in developing neural ectoderm.
TS16: diencephalon	Strong		2B	Most intense neural expression in future diencephalon and telencephalic vesicles.
TS16: telencephalon	Strong		2B	Most intense neural expression in future diencephalon and telencephalic vesicles.

Structure	Level	Pattern	Image	Note
TS17: diencephalon	Strong		2C	Most intense neural expression in future diencephalon and telencephalic vesicles.
TS17: telencephalon	Strong		2C	Most intense neural expression in future diencephalon and telencephalic vesicles.
TS17: nasal epithelium	Strong		2C	Strong expression in developing olfactory system, including nasal epithelium.
TS17: olfactory system	Strong		2C	Strong expression in developing olfactory system, including nasal epithelium.
TS17: Rathke's pouch	Present		2C	Most prominent expression in non-ectodermal tissue was in Rathke's pouch.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Strong	Regionally restricted	2D	diencephalic primordia and developing hippocampus. Expression also in developing cerebral cortex.
TS19: telencephalon	Strong	Regionally restricted	2D	diencephalic primordia and developing hippocampus. Expression also in developing cerebral cortex.

Structure	Level	Pattern	Image	Note
TS22: brainstem	Strong	Regionally restricted	3A	Strong expression in brainstem. Most dense expression in reticular formation, coerulear region and medial vestibular complex.
TS22: medulla oblongata	Strong		3A	Expression preferentially in medullary and pontine regions.
TS22: pons	Strong		3A	Expression preferentially in medullary and pontine regions.
TS22: cerebellum	Strong	Regionally restricted	3A	Dense expression in dorsal cerebellum.

Structure	Level	Pattern	Image	Note
TS22: diencephalon	Present	Regionally restricted	3B	More defined expression in developing diencephalon. Expression in a region anterior and dorsal to mammillary area encompassing the subthalamic nucleus and possibly the medial geniculate body No expression in anterior hypothalamic area, and large parts of dorsal (principal) thalamus.
TS22: zona incerta	Strong		3B
TS22: mammillary area	Absent		3B

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Strong	Graded	3C
TS22: cerebral cortex ventricular layer	Present		3C	Clear gradient of expression between ventricular zone (lower expression) and differentiating cells of intermediate zone (stronger expression).
TS22: cerebral cortex intermediate zone	Strong		3C	Clear gradient of expression between ventricular zone (lower expression) and differentiating cells of intermediate zone (stronger expression).
TS22: hippocampus	Present		3C	Prominent expression in developing hippocampus (archicortex).

Structure	Level	Pattern	Image	Note
TS22: basal ganglia	Present	Widespread		Expression in all parts of basal ganglia, including substantia nigra and striatum.
TS22: caudate-putamen	Present
TS22: globus pallidus	Present
TS22: lateral ganglionic eminence	Present
TS22: cochlear ganglion	Present			Prominent expression in different parts of peripheral nervous system, such as the cochlear ganglia.
TS22: peripheral nervous system	Present			Prominent expression in different parts of peripheral nervous system, such as the cochlear ganglia.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex	Present	Regionally restricted	3D	Moderate to intense expression in cerebral cortex, hippocampus, structures of basal ganglia including striatum and globus pallidus, and diffferent parts of olfactory system.
TS25: amygdala	Present		3D
TS25: reticular thalamic nucleus	Present		3D	Moderate to intense expression in reticular thalamic nucleus.
TS25: hypothalamus lateral zone	Present		3D
TS25: midbrain	Moderate	Regionally restricted	3D	Moderate expression in entire mesencephalon.
TS25: superior colliculus	Strong		3D	Stronger expression in superior colliculus, ventral tegmental area and substantia nigra.
TS25: ventral tegmental area	Strong		3D	Stronger expression in superior colliculus, ventral tegmental area and substantia nigra.
TS25: pons	Present	Regionally restricted	3D	Expression in many pontine regions, such as the coerulear region. Most prominent expression in developing pontine nuclei.
TS25: medulla oblongata	Strong	Regionally restricted	3D	Strong expression in medullary reticular formation, medial vestibular complex and ventral motor structures, such as interior olive and nucleus raphe magnus.

Structure	Level	Pattern	Image	Note
TS25: thalamic nucleus	Present	Regionally restricted		Moderate to intense expression in ventromedial thalamic nucleus.
TS25: spinal cord dorsal horn	Strong			Expression extended down in spinal cord, with intense expression in intermediate and dorsal horn.
TS25: spinal cord intermediate grey	Strong			Expression extended down in spinal cord, with intense expression in intermediate and dorsal horn.
TS25: spinal cord ventral horn	Moderate

Structure	Level	Pattern	Image	Note
TS27: basal ganglia	Strong		4A
TS27: thalamic nucleus	Present	Regionally restricted	4A	Less intense expression in ventromedial thalamic nucleus. More intense expression in developing intralaminar nuclei.
TS27: zona incerta	Strong		4A
TS27: anterior hypothalamic nucleus	Absent		4A
TS27: olfactory system	Present	Widespread	4A	Dense expression in entire olfactory system.
TS27: main olfactory bulb	Present		4A
TS27: nasal epithelium	Present		4A
TS27: olfactory tubercle	Present		4A
TS27: piriform cortex	Present		4A
TS27: mammillary body	Absent		4A

Structure	Level	Pattern	Image	Note
TS27: reticular thalamic nucleus	Present		4B
TS27: accumbens nucleus	Strong		4B

Structure	Level	Pattern	Image	Note
TS27: mammillary body	Absent		4C

Structure	Level	Pattern	Image	Note
TS27: spinal cord	Present		4D

Structure	Level	Pattern	Image	Note
TS27: trigeminal ganglion	Present		4E
TS27: trigeminal nerve mandibular division	Present		4E
TS27: trigeminal nerve maxillary division	Present		4E
TS27: trigeminal nerve ophthalmic division	Present		4E

Structure	Level	Pattern	Image	Note
TS27: nasal epithelium	Present		4F	Expression in nasal epithelium and surrounding structures.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present		5A	Expression in cortical layers 2-6.
TS28: cortical layer III	Present		5A	Expression in cortical layers 2-6.
TS28: cortical layer V	Present		5A	Expression in cortical layers 2-6.
TS28: cortical layer VI	Present		5A	Expression in cortical layers 2-6.
TS28: cortical layer IV	Weak		5A	Weaker expression in layer 4.
TS28: piriform cortex	Present		5A
TS28: hippocampus	Present		5A
TS28: hippocampus CA3	Strong		5A
TS28: dentate gyrus	Present		5A

Structure	Level	Pattern	Image	Note
TS28: amygdala	Present		5B
TS28: basal ganglia	Present		5B
TS28: corpus striatum	Present		5B

Structure	Level	Pattern	Image	Note
TS28: lateral hypothalamic area	Present		5C
TS28: perifornical nucleus	Present		5C
TS28: ventromedial hypothalamic nucleus	Present		5C

Structure	Level	Pattern	Image	Note
TS28: central lateral thalamic nucleus	Present		5D