Gja1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316204 Chen Z, et al., Geranylgeranyl pyrophosphate synthase facilitates the organization of cardiomyocytes during mid-gestation through modulating protein geranylgeranylation in mouse heart. Cardiovasc Res. 2018 Jun 1;114(7):965-978
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7449024
Gene symbol:	Gja1
Gene name:	gap junction protein, alpha 1

Antibody:	Anti-Connexin 43 (3512)
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	Multiple Ggps1 antibodies were described, but the one used in this assay was not detailed. Anti-GGPS1 MGI:7448882 may have been used in this assay.

Results

Specimen 6D Cx43 Control: embryonic day 11.5; Ggps1^tm1Licj/? (more

)
Note: Controls were Cre-negative littermates (fl/fl or fl/+). Double labeled: red - Cx43 (Gja1); green - WGA

Structure	Level	Pattern	Image	Note
TS19: heart	Trace		6D Control Merge (upper) , 6D Control Merge (lower) , 6D Control Cx43

Specimen 6D Cx43 Cre/+: embryonic day 11.5; Ggps1^tm1Licj/Ggps1^tm1Licj, Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (more

)
Note: Conditional mutant. Double labeled: red - Cx43 (Gja1); green - WGA

Structure	Level	Pattern	Image	Note
TS19: heart	Present	Regionally restricted	6D Cre/+ Merge (upper) , 6D Cre/+ Cx43 , 6D Cre/+ Merge (lower)	Most of expression accumulated in cytoplasm.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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