Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:316204 Chen Z, et al., Geranylgeranyl pyrophosphate synthase facilitates the organization of cardiomyocytes during mid-gestation through modulating protein geranylgeranylation in mouse heart. Cardiovasc Res. 2018 Jun 1;114(7):965-978
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7449020
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	anti-alpha-SMA (MA5-11544)
Detection system:	Secondary antibody coupled to Alexa Fluor 488
Assay notes:	Multiple Ggps1 antibodies were described, but the one used in this assay was not detailed. Anti-GGPS1 MGI:7448882 may have been used in this assay.

Results

Specimen 5B E10.5 Control: embryonic day 10.5; Ggps1^tm1Licj/? (more

)
Note: Controls were Cre-negative littermates (fl/fl or fl/+).

Structure	Cell Type	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	cardiac muscle cell	Present		5B E10.5 Control

Specimen 5B E10.5 Cre/+: embryonic day 10.5; Ggps1^tm1Licj/Ggps1^tm1Licj, Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	cardiac muscle cell	Present		5B E10.5 Cre/+

Specimen 5B E11.5 Control: embryonic day 11.5; Ggps1^tm1Licj/? (more

)
Note: Controls were Cre-negative littermates (fl/fl or fl/+).

Structure	Cell Type	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	cardiac muscle cell	Present		5B E11.5 Control

Specimen 5B E11.5 Cre/+: embryonic day 11.5; Ggps1^tm1Licj/Ggps1^tm1Licj, Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	cardiac muscle cell	Present	Regionally restricted	5B E11.5 Cre/+	Cardiomyocytes in compact zone become round and disorganized after E10.5.

Specimen 5B E12.5 Control: embryonic day 12.5; Ggps1^tm1Licj/? (more

)
Note: Controls were Cre-negative littermates (fl/fl or fl/+).

Structure	Cell Type	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	cardiac muscle cell	Present		5B E12.5 Control

Specimen 5B E12.5 Cre/+: embryonic day 12.5; Ggps1^tm1Licj/Ggps1^tm1Licj, Nkx2-5^tm1(cre)Rjs/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Cell Type	Level	Pattern	Image	Note
TS20: compact myocardium	cardiac muscle cell	Present	Regionally restricted	5B E12.5 Cre/+	Cardiomyocytes in compact zone become round and disorganized after E10.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24