Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:229936 Cano E, et al., Extracardiac septum transversum/proepicardial endothelial cells pattern embryonic coronary arterio-venous connections. Proc Natl Acad Sci U S A. 2016 Jan 19;113(3):656-61
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7431189
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Secondary antibody

Results

3B ©

Specimen 3B: embryonic day 17.5; Wt1^tm1.1Ndha/Wt1^tm1.1Ndha (more

)
Note: Double labeled: green - Acta2; red - Pecam1 (assay MGI:7431138).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: coronary artery	smooth muscle cell of the coronary artery	Present		3B
TS25: myocardium layer	cardiac muscle cell	Present		3B

3E ©

Specimen 3E: embryonic day 17.5; Wt1^tm1.1Ndha/Wt1^tm1.1Ndha, Tg(Gata4*G2-cre)#Roja/0 (more

)
Note: Conditional mutant. Double labeled: green - Acta2; red - Pecam1 (assay MGI:7431138).

Structure	Cell Type	Level	Pattern	Image	Note
TS25: myocardium layer	cardiac muscle cell	Present		3E	Normal coronary artery was missing in the mutants, which also displayed a thin ventricular compact myocardium.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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