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Gene Expression Data
Assay Details
Assay
Reference: J:268254 Rich CA, et al., Olfactory ensheathing cells abutting the embryonic olfactory bulb express Frzb, whose deletion disrupts olfactory axon targeting. Glia. 2018 Dec;66(12):2617-2631
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7427402
Gene symbol: Tubb3
Gene name: tubulin, beta 3 class III
Antibody: anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1a: embryonic day 11.5 (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 protein (assay MGI:7427400); Frzb RNA (assay MGI:7427354).
Structure Level Pattern Image Note
TS20: olfactory nerve Present 1c , 1c1
Specimen 1d: embryonic day 13.5 (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 protein (assay MGI:7427400); Frzb RNA (assay MGI:7427354).
Structure Level Pattern Image Note
TS22: main olfactory bulb olfactory nerve layer Present 1f1 , 1g , 1f
TS22: olfactory nerve Present 1g , 1h
Specimen 2a: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 protein (assay MGI:7427400); Frzb RNA (assay MGI:7427354).
Structure Level Pattern Image Note
TS24: main olfactory bulb olfactory nerve layer Present 2b
TS24: olfactory nerve Present 2c1 , 2b
Specimen 2d: embryonic day 16.5 (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 protein (assay MGI:7427400); Npy RNA (assay MGI:7427365).
Structure Level Pattern Image Note
TS24: main olfactory bulb olfactory nerve layer Present 2e
Specimen 3a: embryonic day 13.5 (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 protein (assay MGI:7427400); Frzb RNA (assay MGI:7427354).
Structure Level Pattern Image Note
TS22: main olfactory bulb olfactory nerve layer Present 3d , 3c , 3c1
Specimen 3e: embryonic day 13.5; Sox10tm1Weg/Sox10tm1Weg (more )
Note: Triple labeled: magenta - Tubb3; green - Sox10 reporter (assay MGI:7427366); Frzb RNA (assay MGI:7427354).
Structure Level Pattern Image Note
TS22: main olfactory bulb olfactory nerve layer Present 3g1 , 3g , 3h
Specimen 6a: embryonic day 16.5; Frzbtm1.1Fplu/Frzb+ (more )
Note: Coronal section. Double labeled: magenta - Tubb3; green - Omp (assay MGI:7427364).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium olfactory receptor cell Present 6a , 6d , 6g
Specimen 6b: embryonic day 16.5; Frzbtm1.1Fplu/Frzbtm1.1Fplu (more )
Note: Coronal section. Double labeled: magenta - Tubb3; green - Omp (assay MGI:7427364).
Structure Cell Type Level Pattern Image Note
TS24: olfactory epithelium olfactory receptor cell Present 6e , 6b , 6h The number of olfactory receptor neurons in the null is essentially the same as in the heterozygote.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory