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Gene Expression Data
Assay Details
Assay
Reference: J:225740 Snowball J, et al., Endodermal Wnt signaling is required for tracheal cartilage formation. Dev Biol. 2015 Sep 1;405(1):56-70
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7407319
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-Nkx2.1
Detection system: Secondary antibody
Results
Specimen 4C: embryonic day 13.5 (more )
Note: Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS21: lung epithelium Present 4C
TS21: trachea epithelium Present 4C

Specimen 4D: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS21: lung epithelium Present 4D
TS21: trachea epithelium Present 4D

Specimen 4E: embryonic day 13.5 (more )
Note: Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS21: lung epithelium Present 4E

Specimen 4F: embryonic day 13.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS21: lung epithelium Present 4F

Specimen 5A left control: embryonic day 11.5 (more )
Note: Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS19: trachea epithelium Present 5A left

Specimen 5A left cKO: embryonic day 11.5; Shhtm1(EGFP/cre)Cjt/Shh+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Double labeled: red - Nkx2-1; green - Sox9 (assay MGI:7407287).
Structure Level Pattern Image Note
TS19: trachea epithelium Present 5A middle

Specimen S5A: embryonic day 12.5 (more )
Note: Triple labeled: red - Nkx2-1; green - Cre reporter; blue - Sox9 (assay MGI:7388674).
Structure Level Pattern Image Note
TS20: trachea epithelium Present S5A

Specimen S5B: embryonic day 12.5; Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sor+, Twist2tm1.1(cre)Dor/Twist2+, Wlstm1.1Lan/Wlstm1.1Lan (more )
Note: Conditional mutant. Triple labeled: red - Nkx2-1; green - Cre reporter; blue - Sox9 (assay MGI:7388674).
Structure Level Pattern Image Note
TS20: trachea epithelium Present S5B


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory