About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388341
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Antibody: anti-TTF1 (WRAB-1231)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 7A control: embryonic day 14.5 (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 7A lower control
Specimen 7A -/-: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 7A lower -/- Similar as in the control.
Specimen 9C control: embryonic day 12.5 (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS20: trachea epithelium Present 9C control lower , 9C control upper
Specimen 9C mut: embryonic day 12.5; Ctnnb1tm1Mmt/Ctnnb1+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS20: trachea epithelium Present 9C cKO lower , 9C cKO upper Similar as in the control.
Specimen 9D Ror2 f/f: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D left upper , 9D left lower
Specimen 9D Ror2 cKO: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D middle lower , 9D middle upper Tracheal stenosis was seen.
Specimen 9D Notum 150/150: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: blue - Nkx2-1; red - Acta2 (assay MGI:7388339); green - Sox9 (assay MGI:7388334).
Structure Level Pattern Image Note
TS22: trachea epithelium Present 9D right lower , 9D right upper Tracheal stenosis was seen.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
04/16/2024
MGI 6.23 		The Jackson Laboratory