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Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388339
Gene symbol: Acta2
Gene name: actin alpha 2, smooth muscle, aorta
Antibody: Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A control: embryonic day 16.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 5A control Expressed on the dorsal side.
Specimen 5A -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 5A -/- Reduced expression on the dorsal side and the luminal area of the trachea was decreased.
Specimen 5B control: embryonic day 16.5 (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS24: bronchus smooth muscle bronchial smooth muscle cell Present 5B control
Specimen 5B -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: red - Acta2; green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS24: bronchus smooth muscle bronchial smooth muscle cell Present 5B -/- Smaller luminal area was seen.
Specimen 7A control: embryonic day 14.5 (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 7A lower control
Specimen 7A -/-: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present 7A lower -/-
Specimen 9C control: embryonic day 12.5 (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme smooth muscle cell of trachea Present 9C control upper , 9C control lower
Specimen 9C mut: embryonic day 12.5; Ctnnb1tm1Mmt/Ctnnb1+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme smooth muscle cell of trachea Absent 9C cKO lower , 9C cKO upper
Specimen 9D Ror2 f/f: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 9D left lower , 9D left upper Expressed on the dorsal side.
Specimen 9D Ror2 cKO: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 9D middle upper , 9D middle lower Reduced expression on the dorsal side and the luminal area of the trachea was decreased.
Specimen 9D Notum 150/150: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: red - Acta2; blue - Nkx2-1 (assay MGI:7388341); green - Sox9 (assay MGI:7388334).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme smooth muscle cell of trachea Present Regionally restricted 9D right lower , 9D right upper Reduced expression on the dorsal side and the luminal area of the trachea was decreased.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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