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Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388334
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox9 (AF3075)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A control: embryonic day 16.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme chondroblast Present 5A control
Specimen 5A -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme chondroblast Present 5A -/- Tracheas were stenotic accompanied with mispatterned cartilage extending into the dorsal side of the trachea.
Specimen 5B control: embryonic day 16.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: bronchus cartilage chondroblast Present 5B control
Specimen 5B -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: bronchus cartilage chondroblast Present 5B -/- Smaller luminal area was seen.
Specimen 6C: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; blue - Acta2 (assay MGI:7388340).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present 6C
Specimen 6D: embryonic day 13.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; blue - Acta2 (assay MGI:7388340).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present 6D Cell proliferation in the mesenchyme was similar as in the control.
Specimen 7A control: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 7A lower control Expressed in well-defined mesenchymal condensations.
Specimen 7A -/-: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 7A lower -/- Loss of condensations was seen.
Specimen 9C control: embryonic day 12.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Present 9C control lower , 9C control upper
Specimen 9C mut: embryonic day 12.5; Ctnnb1tm1Mmt/Ctnnb1+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Absent 9C cKO lower , 9C cKO upper
Specimen 9D Ror2 f/f: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D left lower , 9D left upper
Specimen 9D Ror2 cKO: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D middle upper , 9D middle lower Decreased expression compared with the wild-type control.
Specimen 9D Notum 150/150: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D right upper , 9D right lower Trachea was stenotic accompanied with mispatterned cartilage extending into the dorsal side of the trachea.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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