About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:260471 Gerhardt B, et al., Notum attenuates Wnt/beta-catenin signaling to promote tracheal cartilage patterning. Dev Biol. 2018 Apr 1;436(1):14-27
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7388334
Gene symbol: Sox9
Gene name: SRY (sex determining region Y)-box 9
Antibody: anti-Sox9 (AF3075)
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 5A control: embryonic day 16.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme chondroblast Present 5A control
Specimen 5A -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: trachea mesenchyme chondroblast Present 5A -/- Tracheas were stenotic accompanied with mispatterned cartilage extending into the dorsal side of the trachea.
Specimen 5B control: embryonic day 16.5 (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: bronchus cartilage chondroblast Present 5B control
Specimen 5B -/-: embryonic day 16.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS24: bronchus cartilage chondroblast Present 5B -/- Smaller luminal area was seen.
Specimen 6C: embryonic day 13.5 (more )
Note: Double labeled: green - Sox9; blue - Acta2 (assay MGI:7388340).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present 6C
Specimen 6D: embryonic day 13.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Double labeled: green - Sox9; blue - Acta2 (assay MGI:7388340).
Structure Cell Type Level Pattern Image Note
TS21: trachea mesenchyme chondroblast Present 6D Cell proliferation in the mesenchyme was similar as in the control.
Specimen 7A control: embryonic day 14.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present Regionally restricted 7A lower control Expressed in well-defined mesenchymal condensations.
Specimen 7A -/-: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 7A lower -/- Loss of condensations was seen.
Specimen 9C control: embryonic day 12.5 (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Present 9C control lower , 9C control upper
Specimen 9C mut: embryonic day 12.5; Ctnnb1tm1Mmt/Ctnnb1+, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS20: trachea mesenchyme chondroblast Absent 9C cKO lower , 9C cKO upper
Specimen 9D Ror2 f/f: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D left lower , 9D left upper
Specimen 9D Ror2 cKO: embryonic day 14.5; Ror2tm1.1Meg/Ror2tm1.1Meg, Twist2tm1.1(cre)Dor/Twist2+ (more )
Note: Conditional mutant. Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D middle upper , 9D middle lower Decreased expression compared with the wild-type control.
Specimen 9D Notum 150/150: embryonic day 14.5; Notumem150Sinn/Notumem150Sinn (more )
Note: Triple labeled: green - Sox9; blue - Nkx2-1 (assay MGI:7388341); red - Acta2 (assay MGI:7388339).
Structure Cell Type Level Pattern Image Note
TS22: trachea mesenchyme chondroblast Present 9D right upper , 9D right lower Trachea was stenotic accompanied with mispatterned cartilage extending into the dorsal side of the trachea.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory