Reporter:	GFP
Antibody:	anti-GFP (#A11122)
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS21: zeugopod	Strong	Non-Uniform	1A	Expression was particularly strong in the wrist and ankle joints.
TS21: elbow	Absent		1A
TS21: knee	Absent		1A

Structure	Level	Pattern	Image	Note
TS22: zeugopod	Present		1B
TS22: ankle	Strong		1B
TS22: wrist	Strong		1B
TS22: knee	Present		1B
TS22: elbow	Absent		1B

Structure	Level	Pattern	Image	Note
TS23: zeugopod	Present		1C
TS23: ankle	Strong		1C
TS23: wrist	Strong		1C
TS23: knee	Present		1C
TS23: elbow	Absent		1C

Structure	Level	Pattern	Image	Note
TS25: zeugopod	Present		1D
TS25: ankle	Strong		1D
TS25: wrist	Strong		1D
TS25: knee	Present		1D
TS25: elbow	Absent		1D

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	1E	Expression was particularly strong in the wrist.
TS21: elbow	Absent		1E

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	1F	Expression was particularly strong in the ankle.
TS21: knee	Absent		1F

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Non-Uniform	1G
TS22: wrist	Strong		1I , 1G
TS22: elbow	Absent		1J , 1G

Structure	Level	Pattern	Image	Note
TS22: hindlimb	Present	Non-Uniform	1H
TS22: ankle	Strong		1H , 1K
TS22: knee	Present		1L , 1H

Structure	Level	Pattern	Image	Note
TS23: zeugopod	Present	Regionally restricted	1M	Expression was detected along the opposing articular surfaces of the distal zeugopod long bones.
TS23: scapholunate	Present	Regionally restricted	1O Hoxa11eGFP , 1M , 1O merge	Expression was restricted to the outer cells of the scaphoid and lunate in the wrist with a high degree of overlap with Gdf5-lineage cells.
TS23: elbow	Absent		1R merge , 1R Hoxa11egfp , 1M

Structure	Level	Pattern	Image	Note
TS23: zeugopod	Present	Regionally restricted	1N	Expression was detected along the opposing articular surfaces of the distal zeugopod long bones.
TS23: calcaneus	Present	Regionally restricted	1N , 1P merge , 1P Hoxa11egfp	Expression was restricted to the outer cells of the calcaneous and talus in the ankle with a high degree of overlap with Gdf5-lineage cells.
TS23: talus	Present	Regionally restricted	1N , 1P merge , 1P Hoxa11egfp	Expression was restricted to the outer cells of the calcaneous and talus in the ankle with a high degree of overlap with Gdf5-lineage cells.
TS23: knee	Present		1N , 1Q merge , 1Q Hoxa11egfp	Expression strongly overlapped with Gdf5-lineage cells in the knee.

Structure	Level	Pattern	Image	Note
TS23: knee	Present	Regionally restricted	2A merge , 2A Hoxa11eGFP	Expression strongly overlapped with Gdf5-lineage cells in the medial portion of the knee.
TS23: cruciate ligament of knee	Present	Regionally restricted	2A Hoxa11eGFP , 2A merge	The overlap of Hoxa11-gfp with Gdf5-lineage cells prominently characterized the incipient intrajoint anterior and posterior cruciate ligaments.

Structure	Level	Pattern	Image	Note
TS23: knee	Present	Regionally restricted	2B merge , 2B Hoxa11eGFP	Expression strongly overlapped with Gdf5-lineage cells in the central portion of the knee.
TS23: cruciate ligament of knee	Present	Regionally restricted	2B Hoxa11eGFP , 2B merge	The overlap of Hoxa11-gfp with Gdf5-lineage cells prominently characterized the incipient intrajoint anterior and posterior cruciate ligaments.

Structure	Level	Pattern	Image	Note
TS23: knee	Weak	Regionally restricted	2C Hoxa11eGFP , 2C merge	The lateral portion of the knee displayed abundant Gdf5-lineage cells, but minimal if any expression of Hoxa11-gfp.

Structure	Level	Pattern	Image	Note
TS23: knee	Weak	Regionally restricted	2D merge , 2D Hoxa11eGFP	The patella portion of the knee displayed abundant Gdf5-lineage cells, but minimal if any expression of Hoxa11-gfp.
TS23: patella pre-cartilage condensation	Weak	Regionally restricted	2D merge , 2D Hoxa11eGFP	The patella portion of the knee displayed abundant Gdf5-lineage cells, but minimal if any expression of Hoxa11-gfp.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: wrist		Present	Widespread	3A
TS27: synovium	type B synovial cell	Present	Regionally restricted	3A	Expression was detected in the synovial fibroblasts of the wrist.
TS27: articular cartilage	articular chondrocyte	Strong	Regionally restricted	3E , 3C , 3A	There was strong expression in the nascent articular chondrocytes juxtaposed to the emerging synovial joint space.
TS27: epiphyseal plate ossification zone	chondrocyte	Present	Regionally restricted	3A , 3E , 3C	Expression was detected in secondary ossification center/epiphyseal cartilage of the radius.
TS27: proximal carpal bone	chondrocyte	Weak	Regionally restricted	3A , 3E , 3C	Low expression was detected in ossification centers of smaller joint elements.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: ankle		Present	Widespread	3B
TS27: synovium	type B synovial cell	Present	Regionally restricted	3B	Expression was detected in the synovial fibroblasts of the ankle.
TS27: articular cartilage	articular chondrocyte	Strong	Regionally restricted	3F , 3D , 3B	There was strong expression in the nascent articular chondrocytes juxtaposed to the emerging synovial joint space.
TS27: epiphyseal plate ossification zone	chondrocyte	Present	Regionally restricted	3B , 3F , 3D	Expression was detected in secondary ossification center/epiphyseal cartilage of the tibia.
TS27: tarsal bone	chondrocyte	Weak	Regionally restricted	3B , 3D , 3F	Low expression was detected in ossification centers of smaller joint elements.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: joint fibrous capsule		Present		3I
TS28: synovium		Present		3I
TS28: articular cartilage	articular chondrocyte	Strong	Regionally restricted	3I , 3M , 3K	Strong staining characterized mature articular chondrocytes that were present above the tidemark of the DIC imaging.
TS28: bone marrow	stromal cell of bone marrow	Present	Single cells	3I	Expression was detected in cells in marrow/woven bone, likely representing skeletal progenitors.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: joint fibrous capsule		Present		3J
TS28: synovium		Present		3J
TS28: fat pad		Present	Regionally restricted	3J , 3N , 3L	Expression was in the fat pad of the ankle.
TS28: articular cartilage	articular chondrocyte	Strong	Regionally restricted	3N , 3J , 3L	Strong staining characterized mature articular chondrocytes that were present above the tidemark of the DIC imaging.
TS28: bone marrow	stromal cell of bone marrow	Present	Regionally restricted	3J	Expression was detected in cells in marrow/woven bone, likely representing skeletal progenitors.

Structure	Level	Pattern	Image	Note
TS27: tibia	Present	Regionally restricted	4B	Expression was largely confined to the medial tibial plateau. The lateral portion of the plateau exhibited ony a few scattered expressing cells.

Structure	Level	Pattern	Image	Note
TS28: tibia	Present	Regionally restricted	4C	Expression was largely confined to the medial tibial plateau. The lateral portion of the plateau exhibited ony a few scattered expressing cells. The anterior and posterior contours were essentially negative.

Structure	Level	Pattern	Image	Note
TS28: tibia	Present	Regionally restricted	4D	Expression was largely confined to the medial tibial plateau. The lateral portion of the plateau exhibited ony a few scattered expressing cells. The anterior and posterior contours were essentially negative.

Structure	Cell Type	Level	Pattern	Image	Note
TS27: articular cartilage		Weak	Regionally restricted	5B , 5A	Low expression was detected in about 30-40% of nascent articular cartilage of tibia and femur (~6-7 cell layers of prospective articular tissue).
TS27: epiphyseal plate ossification zone	chondrocyte	Strong	Regionally restricted	5A , 5B	Very strong expression was detected in underlying non-articular chondrocytes within the epiphyseal cartilage of tibia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: articular cartilage	chondrocyte	Strong	Scattered	5C , 5D	Strong expression increased rapidly by 2 weeks in knee articular cartilage of tibia and femur .
TS28: epiphyseal plate ossification zone	hypertrophic chondrocyte	Present	Regionally restricted	5C , 5D	High expression was detected in the secondary ossification center/epiphyseal cartilage of the tibia.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: articular cartilage	chondrocyte	Present	Regionally restricted	5E , 5F	High expression was maintained in maturing and mature chondrocytes in knee articular cartilage at postnatal month 1 of tibia and femur above the tidemark and calcified cartilage.
TS28: tibia		Present	Single cells	5F , 5E	Only a few Hoxa11-expressing cells were present in the superficial zone and the calcified cartilage beneath the tidemark.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	5H , 5G	High expression was maintained in knee articular cartilage at postnatal month 2 of tibia above the tidemark and calcified cartilage. In femoral articular cartilage, expression was dramatically reduced in 2 months old animals.
TS28: tibia	Present	Single cells	5G , 5H	Only a few Hoxa11-expressing cells were present in the superficial zone.

Structure	Level	Pattern	Image	Note
TS27: meniscus of knee	Present	Regionally restricted	5K	Expression was detected in posterior meniscus.

Structure	Level	Pattern	Image	Note
TS27: meniscus of knee	Present	Regionally restricted	5L	Expression was detected in anterior meniscus.

Structure	Level	Pattern	Image	Note
TS27: ligament	Present	Regionally restricted	5M	Expression was detected in knee intra joint ligaments, posterior collateral ligament.

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Single cells	5N	Expression declined in posterior meniscus at 2 months of age and was maintained in only a few cells.

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Single cells	5O	Expression declined in anterior meniscus at 2 months of age and was maintained in only a few cells.

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Single cells	5P	Expression declined in knee intra joint ligaments, posterior collateral ligament at 2 months of age and was maintained in only a few cells.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: wrist		Strong	Regionally restricted	6A	Expression pattern resembled that observed at 2 months of age.
TS28: synovium	type B synovial cell	Present		6A
TS28: articular cartilage		Strong	Regionally restricted	6A , 6C , 6D	Expression was strong throughout articular cartilage in elements including scaphoid, lunate, radius and ulna in the wrist consistently above the tidemark.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: ankle		Strong	Regionally restricted	6B	Expression pattern resembled that observed at 2 months of age.
TS28: synovium	type B synovial cell	Present		6B
TS28: fat pad		Present	Regionally restricted	6B	Expression was detected in the fat pad of the ankle.
TS28: articular cartilage		Strong	Regionally restricted	6F , 6B , 6E	Expression was strong throughout articular cartilage in elements including talus and tibia in the ankle consistently above the tidemark.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: knee joint		Weak	Regionally restricted	6G	Expression was dramatically reduced in the knee joint at 6 months.
TS28: meniscus of knee		Absent		6G
TS28: articular cartilage	columnar chondrocyte	Strong	Regionally restricted	6H , 6I , 6G	Strong expression was largely restricted to deep zone columnar chondrocytes above the tidemark and calcified cartilage. Superficial zone cells mostly do not express Hoxa11eGFP. Expression was absent from femoral cartilage.

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Regionally restricted	6J	Expression was maintained in intrajoint ligaments in patterns and levels resembling those at 2 months of age.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	S1A , S1C' , S1E'	High expression was detected in the distal-most region of zeugopod in the forelimb including joint progenitor interzone cells in developing wrist, but not elbow.
TS21: zeugopod	Present	Regionally restricted	S1A , S1C' , S1E'	High expression was detected in the distal-most region of zeugopod in the forelimb including joint progenitor interzone cells in developing wrist, but not elbow.
TS21: elbow	Absent		S1E' , S1A

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	S1D' , S1F' , S1B	High expression was detected in the distal-most region of zeugopod in the hindlimb including joint progenitor interzone cells of the ankle, but not the knee.
TS21: zeugopod	Present	Regionally restricted	S1D' , S1F' , S1B	High expression was detected in the distal-most region of zeugopod in the hindlimb including joint progenitor interzone cells of the ankle, but not the knee.
TS21: knee	Absent		S1B , S1F'

Structure	Level	Pattern	Image	Note
TS22: forelimb	Present	Regionally restricted	S1G	High expression was detected in the distal-most region of zeugopod in the forelimb.
TS22: zeugopod	Present	Regionally restricted	S1G	High expression was detected in the distal-most region of zeugopod in the forelimb.
TS22: wrist	Present		S1I' , S1G
TS22: elbow	Absent		S1K' , S1G

Structure	Level	Pattern	Image	Note
TS22: hindlimb	Present	Regionally restricted	S1H	High expression was detected in the distal-most region of zeugopod in the hindlimb.
TS22: zeugopod	Present	Regionally restricted	S1H	High expression was detected in the distal-most region of zeugopod in the hindlimb.
TS22: ankle	Present		S1H , S1J'
TS22: knee	Present		S1H , S1L'

Structure	Level	Pattern	Image	Note
TS27: elbow	Present	Single cells	S3A , S3C , S3E

Structure	Level	Pattern	Image	Note
TS28: elbow	Absent		S3B , S3F , S3D

Structure	Level	Pattern	Image	Note
TS28: hip	Absent		S3G , S3H , S3I

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Regionally restricted	S4A	Expression was detected in posterior meniscus.

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Regionally restricted	S4B	Expression was detected in anterior meniscus.

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Regionally restricted	S4C	Expression was detected in knee intra joint ligaments, posterior collateral ligament.

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Regionally restricted	S4D	Expression was detected in posterior meniscus.

Structure	Level	Pattern	Image	Note
TS28: meniscus of knee	Present	Regionally restricted	S4E	Expression was detected in anterior meniscus.

Structure	Level	Pattern	Image	Note
TS28: ligament	Present	Regionally restricted	S4F	Expression was detected in knee intra joint ligaments, posterior collateral ligament.