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Gene Expression Data
Assay Details
Assay
Reference: J:282343 Sagai T, et al., SHH signaling mediated by a prechordal and brain enhancer controls forebrain organization. Proc Natl Acad Sci U S A. 2019 Nov 19;116(47):23636-23642
Assay type: RNA in situ
MGI Accession ID: MGI:7345906
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe221
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Fixative for whole mount stained embryos was 4% paraformaldehyde containing 0.2% glutaraldehyde.
Results
Specimen 3B: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: axial mesoderm Present Regionally restricted 3B , 3D Expression is in the prechordal plate.
TS13: future prosencephalon Present Regionally restricted 3D , 3B Expression is in the ventral midline and the rostral tip of the diencephalon.
Specimen 3C: embryonic day 8.5; Rr77em1Tshir/Rr77em1Tshir (more )
Structure Level Pattern Image Note
TS13: axial mesoderm Present Regionally restricted 3E , 3C This mutant has a reduced domain of expression.
TS13: future prosencephalon Present Regionally restricted 3E , 3C This mutant has a reduced domain of expression and is absent in the diencephalon.
Specimen 3F: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present 3F
TS17: telencephalon Present 3F
TS17: midbrain Present Regionally restricted 3F Expression is in the ventral midbrain.
Specimen 3G: embryonic day 10.5; Rr77em1Tshir/Rr77em1Tshir (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 3G Expression remained in the caudal diencephalon including the zona limitans intrathalamica but is lost in the rostral diencephalon.
TS17: telencephalon Absent 3G
Specimen 3H: embryonic day 10.5; Del(5Rr78-Rr77)3Tshir/Del(5Rr78-Rr77)3Tshir (more )
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 3H Expression remained in the caudal diencephalon including the zona limitans intrathalamica but is lost in the rostral diencephalon.
TS17: telencephalon Absent 3H
Specimen 3I: embryonic day 10.5; Del(5Rr78-Rr116)2Tshir/Del(5Rr78-Rr116)2Tshir (more )
Structure Level Pattern Image Note
TS17: diencephalon Weak Regionally restricted 3I Expression included the rostral diencephalon.
TS17: telencephalon Absent 3I
Specimen S5A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: diencephalon Present S5C , S5A
TS17: telencephalon Present S5C , S5A
Specimen S5B: embryonic day 10.5; Del(5Rr78-Rr116)2Tshir/Del(5Rr78-Rr116)2Tshir (more )
Structure Level Pattern Image Note
TS17: diencephalon Present S5B , S5D Expression included the rostral diencephalon.
TS17: telencephalon Absent S5D , S5B
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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