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Gene Expression Data
Assay Details
Assay
Reference: J:306393 Ofek S, et al., Notch signaling is a critical initiator of roof plate formation as revealed by the use of RNA profiling of the dorsal neural tube. BMC Biol. 2021 Apr 23;19(1):84
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7338980
Gene symbol: Notch1
Gene name: notch 1
Antibody: anti-Cleaved Notch1 (Val1744) (D3B8) (#4147)
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: The antibody used recognizes the activated form of the protein.
Results
Specimen 3A/B: embryonic day 9.75 (more )
Structure Level Pattern Image Note
TS16: neural tube Weak 3A , 3B Almost undetectable.
Specimen 3C/D: embryonic day 9.75; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS16: neural tube Ambiguous 3C , 3D
Specimen 3E/F: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Widespread 3F , 3E
TS17: neural tube roof plate Absent 3E , 3F
Specimen 3G/H: embryonic day 10.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: neural tube lateral wall Present Regionally restricted 3H , 3G Diminished expression compared with the wild-type. Expression was specifically absent in the dorsal, while still present in more ventral domains.
Specimen 3I/J: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present Widespread 3J , 3I
TS19: neural tube roof plate Absent 3I , 3J
Specimen 3K/L: embryonic day 11.5; Mib1tm2Kong/Mib1tm2Kong, H2az2Tg(Wnt1-cre)11Rth/H2az2+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present Regionally restricted 3L , 3K Diminished expression compared with the wild-type. Expression was specifically absent in the dorsal, while still present in more ventral domains.
Specimen 3AA: embryonic day 11.5 (more )
Note: Double labeled: red - Notch1; green - Dll1 (assay MGI:7338981).
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 3AA The dorsal limit of expression coincided with that of Dll1.
TS19: neural tube roof plate Absent 3AA
Specimen 3CC/DD: embryonic day 11.5 (more )
Note: Double labeled: red - Notch1; green/blue - Gdf7 (assay MGI:7339025).
Structure Level Pattern Image Note
TS19: neural tube lateral wall Present 3DD , 3CC Side-by-side expression of Notch1 and Gdf7 was seen, with no apparent overlap.
TS19: neural tube roof plate Absent 3CC , 3DD
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory