Results
|
Specimen
1A:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
1A
|
Mainly expressed in inner pillar cells in the middle and basal turns of the organ of Corti, and no expression was observed in the apical turn.
|
Specimen
1B E15.5 Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlear duct epithelium |
Present |
Regionally restricted |
1B-1-4
,
1B-1-5
,
1B-1-1
|
Mainly expressed in one row of Sox2-positive progenitor cells in the middle and basal turns of the organ of Corti, and no expression was observed in the apical turn.
|
Specimen
1B E15.5 Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlear duct epithelium |
Present |
Regionally restricted |
1B-2-4
,
1B-2-1
,
1B-2-5
|
Mainly expressed in one row of Sox2-positive progenitor cells in the middle and basal turns of the organ of Corti, and no expression was observed in the apical turn.
|
Specimen
1B E15.5 Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlear duct epithelium |
Present |
Regionally restricted |
1B-3-4
,
1B-3-1
,
1B-3-5
|
In the basal turn, expressed in one row of Sox2-positive progenitor cells between the inner hair cells and outer hair cells.
|
Specimen
1B E18.5 Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
1B-4-4
,
1B-4-5
,
1B-4-1
|
Expressed in inner pillar cells in the middle and basal turns. Expression was increased compared to that of E15.5. No expression was observed in the apical turn.
|
Specimen
1B E18.5 Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
1B-5-1
,
1B-5-5
,
1B-5-4
|
Expressed in inner pillar cells in the middle and basal turns. Expression was increased compared to that of E15.5.
|
Specimen
1B E18.5 Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
1B-6-1
,
1B-6-4
,
1B-6-5
|
Expressed in inner pillar cells in the middle and basal turns. Expression was increased compared to that of E15.5.
|
Specimen
2A E15.5 Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
2A E15.5 Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
2A E15.5 Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
2A E18.5 Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
organ of Corti supporting cell |
Present |
Regionally restricted |
2A-4-1
,
2A-4-5
,
2A-4-4
|
Expressed in one row of supporting cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
2A E18.5 Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
organ of Corti supporting cell |
Present |
Regionally restricted |
2A-5-1
,
2A-5-5
,
2A-5-4
|
Expressed in one row of supporting cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
2A E18.5 Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
organ of Corti supporting cell |
Present |
Regionally restricted |
2A-6-1
,
2A-6-4
,
2A-6-5
|
Expressed in one row of supporting cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
3A:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
Present |
Regionally restricted |
3A
|
Expressed in one row of supporting cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
3D section Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
3D section Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
3D-2-5
,
3D-2-1
,
3D-2-4
|
Expressed only in the inner pillar cells in the middle and basal turns. Increased expression compared with that of E18.5.
|
Specimen
3D section Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
3D-3-1
,
3D-3-4
,
3D-3-5
|
Expressed only in the inner pillar cells in the middle and basal turns. Increased expression compared with that of E18.5.
|
Specimen
3D wholemount Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
3D wholemount Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
3D-5-1
,
3D-5-4
,
3D-5-5
|
Expressed only in the inner pillar cells in the middle and basal turns. Increased expression compared with that of E18.5.
|
Specimen
3D wholemount Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 1
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
3D-6-4
,
3D-6-5
,
3D-6-1
|
Expressed only in the inner pillar cells in the middle and basal turns. Increased expression compared with that of E18.5.
|
Specimen
4 Apical P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
4 Apical P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
4_Apical-6
,
4_Apical-5
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
4_Apical-6
,
4_Apical-5
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
Specimen
4 Apical P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
4 Middle P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
4 Middle P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
4_Middle-5
,
4_Middle-6
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
4_Middle-5
,
4_Middle-6
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
Specimen
4 Middle P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
4 Basal P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
4 Basal P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
4_Basal-6
,
4_Basal-5
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
4_Basal-6
,
4_Basal-5
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P3.
|
Specimen
4 Basal P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Apical P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
5A-1-1
,
5A-1-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
5A-1-1
,
5A-1-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
5A Apical P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Apical P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Middle P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
5A-2-1
,
5A-2-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
5A-2-1
,
5A-2-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
5A Middle P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Middle P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Basal P7:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 7
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
5A-3-1
,
5A-3-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
TS28: cochlear duct epithelium |
internal pillar cell of cochlea |
Present |
Regionally restricted |
5A-3-1
,
5A-3-2
|
Expressed only in the inner pillar and inner border cells in the middle and basal turns. No expression was observed in the apical turn.
|
Specimen
5A Basal P10:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 10
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
5A Basal P15:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 15
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Lgr6; red - Sox2 (assay MGI:7335247).
|
Specimen
6A P20 upper:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
6A P20 lower:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 20
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
6A P30:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 30
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Specimen
S1 Apical:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
S1_Apical-5
,
S1_Apical-1
,
S1_Apical-4
|
Expressed only in the inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P1.
|
Specimen
S1 Middle:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
S1_Middle-4
,
S1_Middle-5
,
S1_Middle-1
|
Expressed only in the inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P1.
|
Specimen
S1 Basal:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
postnatal day 3
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Lgr6; red - Sox2 (assay MGI:7335247); white - Myo7a (assay MGI:7335961).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS27: cochlear duct epithelium |
border cell of cochlea |
Present |
Regionally restricted |
S1_Basal-4
,
S1_Basal-5
,
S1_Basal-1
|
Expressed only in the inner border cells in the middle and basal turns. No expression was observed in the apical turn. Decreased expression compared with that of P1.
|
Specimen
not shown:
(close )
Genetic Background:
|
B6.129P2-Lgr6tm2.1(cre/ERT2)Cle/J
|
Mutant Allele(s):
|
Lgr6tm2.1(cre/ERT2)Cle/Lgr6+
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cochlea |
Absent |
|
|
No positive cells in the organ of Corti.
|
|