Calca Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:308612 Deal KK, et al., Altered sacral neural crest development in Pax3 spina bifida mutants underlies deficits of bladder innervation and function. Dev Biol. 2021 Aug;476:173-188
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:7331380
Gene symbol:	Calca
Gene name:	calcitonin/calcitonin-related polypeptide, alpha

Antibody:

anti-CGRP (C8198)

Results

6A Pax3+/+ CGRP ©

6A Pax3+/+ Merge ©

Specimen 6A Pax3+/+: embryonic day 14.5; Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Calca; blue - Elavl3 (assay MGI:7331367) and Elavl4 (assay MGI:7331372); red - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: pelvic ganglion	sensory neuron	Present		6A Pax3+/+ Merge , 6A Pax3+/+ CGRP

6A Pax3^Sp-d/Sp-d CGRP ©

6A Pax3^Sp-d/Sp-d Merge ©

Specimen 6A Pax3^Sp-d/Sp-d: embryonic day 14.5; Pax3^Sp-d/Pax3^Sp-d, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Calca; blue - Elavl3 (assay MGI:7331367) and Elavl4 (assay MGI:7331372); red - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: pelvic ganglion	sensory neuron	Absent		6A Pax3^Sp-d/Sp-d CGRP , 6A Pax3^Sp-d/Sp-d Merge

6A Pax3^Sp-d/+ CGRP ©

6A Pax3^Sp-d/+ Merge ©

Specimen 6A Pax3^Sp-d/+: embryonic day 14.5; Pax3^Sp-d/Pax3⁺, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Calca; blue - Elavl3 (assay MGI:7331367) and Elavl4 (assay MGI:7331372); red - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: pelvic ganglion	sensory neuron	Present		6A Pax3^Sp-d/+ CGRP , 6A Pax3^Sp-d/+ Merge

6A Pax3^+/delta5 CGRP ©

6A Pax3^+/delta5 Merge ©

Specimen 6A Pax3^+/delta5: embryonic day 14.5; Pax3^tm1.1Sjc/Pax3⁺, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Calca; blue - Elavl3 (assay MGI:7331367) and Elavl4 (assay MGI:7331372); red - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: pelvic ganglion	sensory neuron	Present		6A Pax3^+/delta5 CGRP , 6A Pax3^+/delta5 Merge

6A Pax3^Sp-d/delta5 CGRP ©

6A Pax3^Sp-d/delta5 Merge ©

Specimen 6A Pax3^Sp-d/delta5: embryonic day 14.5; Pax3^Sp-d/Pax3^tm1.1Sjc, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant. Triple labeled: green - Calca; blue - Elavl3 (assay MGI:7331367) and Elavl4 (assay MGI:7331372); red - Cre reporter.

Structure	Cell Type	Level	Pattern	Image	Note
TS22: pelvic ganglion	sensory neuron	Present		6A Pax3^Sp-d/delta5 Merge , 6A Pax3^Sp-d/delta5 CGRP

8A ©

8C ©

Specimen 8A Pax3+/+: embryonic day 14.5; Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: nerve of bladder	Present	Regionally restricted	8A , 8C	Expression was detected in nerve fibers in the anterior and posterior bladder, but not in the bladder dome.

Specimen not shown Pax3^Sp-d/Sp-d: embryonic day 14.5; Pax3^Sp-d/Pax3^Sp-d, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: nerve of bladder	Absent

Specimen not shown Pax3^Sp-d/+: embryonic day 14.5; Pax3^Sp-d/Pax3⁺, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: nerve of bladder	Present	Regionally restricted		Expression was detected in nerve fibers in the anterior and posterior bladder.

Specimen not shown Pax3^+/delta5: embryonic day 14.5; Pax3^tm1.1Sjc/Pax3⁺, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: nerve of bladder	Weak	Regionally restricted		In the anterior bladder, immunopositive nerve fiber density trended lower. Posterior bladder nerve fiber density did not differ.

Specimen not shown Pax3^Sp-d/delta5: embryonic day 14.5; Pax3^Sp-d/Pax3^tm1.1Sjc, Tg(Sox10-cre)1Sout/0, Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: nerve of bladder	Present	Regionally restricted		Expression was detected in nerve fibers in the anterior and posterior bladder.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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