Gene Expression Image Detail

Reference

Figure

ID

J:308612 Deal KK, et al., Dev Biol. 2021 Aug;476:173-188

8

MGI:7327179

Image

Caption

(A) Example image of nerve fiber quantitation in a sagittal immunostained cryosection through Pax3<+/+> 14.5 dpc bladder. Sections selected for presence of the bladder-ureteral reflection (BUR) and the pubic symphysis (PS) provided comparable anatomical planes. Pixel density in circular selections (blue) at anterior (a), posterior (p), and bladder dome (bd) were quantified. Directionality of anterior (A), dorsal (D), ventral (V), and posterior (P) are indicated. (B) Plot of CGRP + nerve fiber density across genotype classes. Each dot represents average values for independent embryos measured from five pelvic ganglia sections. Average +/-SEM (red lines). (C) Image illustrating measurement of the extent of bladder innervation as a function of overall bladder length. Perpendicular line drawn from the BUR-PS axis midpoint (orange line) to the bladder dome (blue line) defines bladder length compared to the furthest extent of CGRP + fibers in the anterior bladder wall (white line). (D) Plot of CGRP+/bladder length ratio for all Pax3 genotype classes. Each dot represents one animal measured from five sections. Average +/-SEM (red lines). Abbreviations: BUR, bladder-ureteral reflection; PS, pubic symphysis.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2021.03.024 Dev Biol 476: 173-188, Deal KK; Chandrashekar AS; Beaman MM; Branch MC; Buehler DP; Conway SJ; Southard-Smith EM, Altered sacral neural crest development in Pax3 spina bifida mutants underlies deficits of bladder innervation and function. Copyright 2021.

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:7331380 (Calca)
C	MGI:7331380 (Calca)