Probe:	Nodal cDNA8
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS12: node	Present		2A	Left-sided asymmetry was seen in 49 out of 69 embryos (summarized in 5A).
TS12: lateral plate mesoderm	Present	Regionally restricted	2A	Expressed in the left side (85 out of 104 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2B	Left-sided asymmetry was seen in 29 out of 39 embryos (summarized in 5A).
TS12: lateral plate mesoderm	Present	Regionally restricted	2B	Expressed in the left side (67 out of 82 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2C
TS12: lateral plate mesoderm	Absent		2C	Expression in the left side was extinguished at the 6-7 somite stage (19 out of 28 embryos with no lateral plate mesoderm, summarized in 2D).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2E	Symmetric expression (14 out of 26 embryos, summarized in 5B).
TS12: lateral plate mesoderm	Absent		2E	26 out of 30 embryos.

Structure	Level	Pattern	Image	Note
TS12: node	Present		2F	Symmetric expression (11 out of 19 embryos, summarized in 5B).
TS12: lateral plate mesoderm	Present	Regionally restricted	2F	Expressed in the right side (2 out of 31 embryos).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		2G	Bilateral expression (9 out of 17 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2I	Symmetric expression (4 out of 11 embryos, summarized in 5C).
TS12: lateral plate mesoderm	Present		2I	Bilateral expression (15 out of 20 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2J	Symmetric expression (6 out of 8 embryos, summarized in 5C).
TS12: lateral plate mesoderm	Present		2J	Bilateral expression (13 out of 16 embryos).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		2K	Bilateral expression (1 out of 2 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2M
TS12: lateral plate mesoderm	Present		2M	Bilateral expression (2 out of 4 embryos).

Structure	Level	Pattern	Image	Note
TS12: node	Present		2N
TS12: lateral plate mesoderm	Present		2N	Bilateral expression (2 out of 3 embryos).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present		2O	Bilateral expression (1 out of 2 embryos).

Structure	Level	Pattern	Image	Note
TS11: node	Present		5A	Left-sided asymmetry was seen in 20 out of 45 embryos.
TS11: lateral plate mesoderm	Present	Regionally restricted	5A	Expressed in the left side.

Structure	Level	Pattern	Image	Note
TS11: node	Present		5B	Symmetric expression (14 out of 31 embryos).
TS11: lateral plate mesoderm	Absent		5B

Structure	Level	Pattern	Image	Note
TS11: node	Present		5C	Right-sided asymmetry was seen (5 out of 16 embryos).
TS11: lateral plate mesoderm	Present		5C	Bilateral expression, with more expression on the left side.

Structure	Level	Pattern	Image	Note
TS12: node	Present		7C
TS12: lateral plate mesoderm	Present		7C	Expression in the left side.

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent		7F
TS12: node	Trace		7F	Expression was weakened such that any asymmetry could not be recorded.

Structure	Level	Pattern	Image	Note
TS11: node	Present			19 out of 45 embryos with symmetric expression; 6 with right-sided asymmetry (summarized in 5A).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			19 out of 104 embryos with no lateral plate mesoderm, summarized in 2D.
TS12: node	Present			15 out of 69 embryos with symmetric expression; 5 with right-sided asymmetry; 49 with left-sided asymmetry (summarized in 5A).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			15 out of 82 embryos with no lateral plate mesoderm, summarized in 2D.
TS12: node	Present			9 out of 39 embryos with symmetric expression; 1 with right-sided asymmetry; 29 with left-sided asymmetry (summarized in 5A).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		9 out of 28 embryos with expression only on the left side, summarized in 2D.

Structure	Level	Pattern	Image	Note
TS11: node	Present			13 out of 31 embryos with left-sided asymmetry; 4 with right-sided asymmetry (summarized in 5B).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		There were 2 out of 30 embryos showed expression only on the left side; 2 on the right side (summarized in 2H).
TS12: node	Present			9 out of 26 embryos with left-sided asymmetry; 3 with right-sided asymmetry; 14 with symmetric expression (summarized in 5B).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present			There were 5 out of 31 embryos showed expression only on the left side; 2 on the right side; 7 on both sides (summarized in 2H).
TS12: node	Present			5 out of 19 embryos with left-sided asymmetry; 3 with right-sided asymmetry; 11 with symmetric expression (summarized in 5B).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		There were 3 out of 17 embryos showed expression only on the left side; 3 on the right side (summarized in 2H).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			There were 2 out of 17 embryos showed no expression (summarized in 2H).

Structure	Level	Pattern	Image	Note
TS11: node	Present			2 out of 16 embryos with left-sided asymmetry; 9 with symmetric expression (summarized in 5C).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			There were 3 out of 20 embryos showed no expression (summarized in 2L).
TS12: node	Present			2 out of 11 embryos with left-sided asymmetry; 5 with right-sided asymmetry; 4 with symmetric expression (summarized in 5C).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		2 out of 20 embryos expressed only on the right side (summarized in 2L).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		2 out of 16 embryos expressed only on the left side (summarized in 2L).
TS12: node	Present			2 out of 8 embryos with left-sided asymmetry; 6 with symmetric expression (summarized in 5C).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			1 out of 16 embryos with no expression (summarized in 2L).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			1 out of 16 embryos with no expression (summarized in 2L).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		1 out of 4 embryos expressed only on the right side (summarized in 2P).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			1 out of 4 embryos with no expression (summarized in 2P).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Present	Regionally restricted		1 out of 3 embryos expressed only on the right side (summarized in 2P).

Structure	Level	Pattern	Image	Note
TS12: lateral plate mesoderm	Absent			1 out of 2 embryos with no expression (summarized in 2P).