Gene Expression Data
Assay Details
|
Assay
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|
Reference:
|
J:255320
Pederick DT, et al., Abnormal Cell Sorting Underlies the Unique X-Linked Inheritance of PCDH19 Epilepsy. Neuron. 2018 Jan 3;97(1):59-66.e5
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|
Assay type:
|
In situ reporter (knock in)
|
|
MGI Accession ID:
|
MGI:7329910
|
|
Gene symbol:
|
Pcdh19
|
|
Gene name:
|
protocadherin 19
|
|
|
Results
|
Specimen
2D HA-FLAG/beta-Geo:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more  )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
2D HA-FLAG/beta-Geo:
(close  )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex intermediate zone |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
,
2D HA-FLAG/beta-Geo
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
| TS22: cerebral cortex ventricular layer |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
,
2D HA-FLAG/beta-Geo
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
| TS22: cortical plate |
Present |
Regionally restricted |
2D HA-FLAG/beta-Geo X-GAL
,
2D HA-FLAG/beta-Geo
|
Protein expressing and null cells form a complementary and non-overlapping alternating pattern from the ventricular zone to the cortical plate.
|
Specimen
2D HA-FLAG/+:
embryonic day 14.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
2D HA-FLAG/+:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2G HA-FLAG/+:
postnatal day 7; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
2G HA-FLAG/+:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2G HA-FLAG/beta-Geo:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Present |
Regionally restricted |
2G HA-FLAG/beta-Geo
|
There were small interspersed patches of HA-positive and HA-negative staining.
|
Specimen
S2D:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2F left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2F right panel top left:
postnatal day 7; Pcdh19em1Pqt/Y
(more )
Note: Double labeled: green - Pcdh19; magenta - Bcl11b (assay MGI:7329937).
Specimen
S2F right panel top left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
postnatal day 7
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Bcl11b (assay MGI:7329937).
|
Specimen
S2H 13.5:
embryonic day 13.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
S2H 13.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2H 14.5 left:
embryonic day 14.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
S2H 14.5 left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2H 14.5 middle:
embryonic day 14.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
S2H 14.5 middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2H 14.5 right:
embryonic day 14.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
S2H 14.5 right:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2H 15.5:
embryonic day 15.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Specimen
S2H 15.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
S2I 13.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
Regionally restricted |
S2I E13.5
|
There are areas of segregated positive and negative cells.
|
| TS21: ganglionic eminence |
Present |
Regionally restricted |
S2I E13.5
|
There are areas of segregated positive and negative cells.
|
Specimen
S2I 14.5 left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2I E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2I E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2I E14.5 left
|
There are areas of segregated positive and negative cells.
|
Specimen
S2I 14.5 middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2I E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2I E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2I E14.5 middle
|
There are areas of segregated positive and negative cells.
|
Specimen
S2I 14.5 right:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2I E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2I E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2I E14.5 right
|
There are areas of segregated positive and negative cells.
|
Specimen
S2I 15.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
S2I E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: ganglionic eminence |
Present |
Regionally restricted |
S2I E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: hippocampus |
Present |
Regionally restricted |
S2I E15.5
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 13.5:
embryonic day 13.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
S2J 13.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: cerebral cortex |
Present |
Regionally restricted |
S2J E13.5
|
There are areas of segregated positive and negative cells.
|
| TS21: ganglionic eminence |
Present |
Regionally restricted |
S2J E13.5
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 left:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
S2J 14.5 left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 left
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 middle:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
S2J 14.5 middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 middle
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 14.5 right:
embryonic day 14.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
S2J 14.5 right:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebral cortex |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: ganglionic eminence |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
| TS22: hippocampus |
Present |
Regionally restricted |
S2J E14.5 right
|
There are areas of segregated positive and negative cells.
|
Specimen
S2J 15.5:
embryonic day 15.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
Specimen
S2J 15.5:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19 HA reporter; magenta - Pcdh19 beta-Geo reporter (assay MGI:7329912).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: ganglionic eminence |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
| TS23: hippocampus |
Present |
Regionally restricted |
S2J E15.5
|
There are areas of segregated positive and negative cells.
|
Specimen
S3A left:
embryonic day 10.5; Pcdh19em1Pqt/Y
(more )
Note: Double labeled with phalloidin (magenta).
Specimen
S3A left:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled with phalloidin (magenta).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain ventricular layer |
Present |
|
S3A left top
,
S3A left bottom
|
Expression is at all cell-cell boundaries.
|
Specimen
S3A middle:
embryonic day 10.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Note: Double labeled with phalloidin (magenta).
Specimen
S3A middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled with phalloidin (magenta).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain ventricular layer |
Present |
Regionally restricted |
S3A middle top
,
S3A middle bottom
|
Expression is at boundaries between HA-positive and HA-negative cells.
|
Specimen
S3A right:
embryonic day 10.5; Pcdh19tm1Tac/ Pcdh19em1Pqt
(more )
Note: Double labeled with phalloidin (magenta).
Specimen
S3A right:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled with phalloidin (magenta).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: brain ventricular layer |
Present |
Regionally restricted |
S3A right top
,
S3A right bottom
|
Expression is absent at boundaries between HA-positive and HA-negative cells.
|
Specimen
S3C:
embryonic day 8.5; Pcdh19em1Pqt/Y
(more )
Note: Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
Specimen
S3C:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3D top:
embryonic day 9.5; Pcdh19em1Pqt/Y
(more )
Note: Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
Specimen
S3D top:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3D middle:
embryonic day 9.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Note: Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
Specimen
S3D middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3D bottom:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3E top:
embryonic day 10.5; Pcdh19em1Pqt/Y
(more )
Note: Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
Specimen
S3E top:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Y
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3E middle:
embryonic day 10.5; Pcdh19em1Pqt/Pcdh19 +
(more )
Note: Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
Specimen
S3E middle:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19em1Pqt/Pcdh19+
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
Specimen
S3E bottom:
(close )
|
Genetic Background:
|
involves: 129S/SvEv * C57BL/6
|
|
Mutant Allele(s):
|
Pcdh19tm1Tac/Pcdh19em1Pqt
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Female
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Double labeled: green - Pcdh19; magenta - Sox3 (assay MGI:7329952).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
S3E bottom
,
S3E bottom left
|
There are areas of segregated positive and negative cells.
|
|
Analysis Tools