Antibody:	anti-Lmx1b
Detection system:	Secondary antibody coupled to Alexa Fluor 546

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8A WT FL	Expressed in the nuclei of the dorsal mesenchyme, a pattern that extended to within tens of microns of the limb tip.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8A WT HL	Expressed in the nuclei of the dorsal mesenchyme, a pattern that extended to within tens of microns of the limb tip.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8A Wnt7a-/- FL	Markedly reduced expression in the distal ~500 um of the limb.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8A Wnt7a-/- HL	Markedly reduced expression in the distal ~500 um of the limb.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8A Wnt7a-/-7b+/- FL	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8A Wnt7a-/-7b+/- HL	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8A Reck FL-1	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8A Reck FL-2	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8A Reck HL-1	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8A Reck HL-2	Markedly reduced expression with variable loss at different depths within the limb and small clusters of positive nuclei often observed within distal limb regions.

Structure	Level	Pattern	Image	Note
TS21: forelimb mesenchyme	Present		8B upper left	Expressed in the nuclei.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8B upper middle	Loss of expression was observed in a spatially patchy distribution.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8B upper right	Loss of expression was observed in a spatially patchy distribution.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8C Wnt7a-/- FL	Loss of expression was observed in a spatially patchy distribution. More limited loss compared with the other two mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Wnt7a-/- HL-1	Loss of expression was observed in a spatially patchy distribution. More limited loss compared with the other two mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Wnt7a-/- HL-2	Loss of expression was observed in a spatially patchy distribution. More limited loss compared with the other two mutants.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8C Wnt7a-/-7b+/- FL	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Wnt7a-/-7b+/- HL-1	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Wnt7a-/-7b+/- HL-2	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8C Reck FL-1	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	8C Reck FL-2	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Reck HL-1	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Reck HL-2	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Reck HL-3	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Reck HL-4	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS21: foot mesenchyme	Present	Regionally restricted	8C Reck HL-5	Loss of expression was observed in a spatially patchy distribution. More severe loss compared with the Wnt7a-/- mutants.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present		S4A left lower , S4A left upper

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present		S4A right lower , S4A right upper

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	S4B FL left upper , S4B FL left lower	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	S4B FL right upper , S4B FL right lower	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present	Regionally restricted	S4B HL left lower , S4B HL left upper	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present	Regionally restricted	S4B HL right lower , S4B HL right upper	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	S4C FL left lower , S4C FL left upper	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud mesenchyme	Present	Regionally restricted	S4C FL right upper , S4C FL right lower	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present	Regionally restricted	S4C HL left upper , S4C HL left lower	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present	Regionally restricted	S4C HL right upper , S4C HL right lower	Spatially heterogeneous expression with truncation of the limb bud on the side opposite the thumb.